MiR23b和Sp1在卵巢子宫内膜异位症中的表达及其意义

发布时间：2018-04-29 11:57

本文选题：miRb + Sp　；参考：《中南大学学报(医学版)》2017年10期

【摘要】：目的:检测miR23b和特异蛋白1(specific protein 1,Sp1)在卵巢子宫内膜异位症中的表达和分布情况,探讨二者在卵巢子宫内膜异位症发生发展中的作用及意义。方法:qPCR检测miR23b和Sp1 mRNA在异位内膜、在位内膜、正常内膜中的表达水平;免疫组织化学和Western印迹检测Sp1蛋白在异位内膜、在位内膜、正常内膜的表达和分布情况。结果:MiR23b mRNA在异位内膜、在位内膜、正常内膜组中的表达水平依次增加(P0.05)。Sp1在子宫内膜间质细胞、腺上皮细胞中均有表达,主要表达在细胞核,少量表达在胞质中;Sp1 mRNA和蛋白在异位内膜、在位内膜、正常内膜中的表达均依次降低(P0.05)。MiR23b与Sp1 mRNA表达呈负相关(r= 0.526,P0.05)。结论:MiR23b和Sp1与卵巢子宫内膜异位症的发生发展密切相关,二者可能通过相互拮抗,共同参与异位病灶的形成。
[Abstract]:Objective: to detect the expression and distribution of miR23b and specific protein 1(specific protein 1 / Sp1 in ovarian endometriosis and to explore the role and significance of them in the occurrence and development of ovarian endometriosis. Methods the expression and distribution of miR23b and Sp1 mRNA in ectopic, eutopic and normal endometrium were detected by 1: qPCR, and the expression and distribution of Sp1 protein in ectopic, eutopic and normal endometrium were detected by immunohistochemistry and Western blot. Results the expression of mRNA in ectopic endometrium, eutopic endometrium, normal endometrium and normal endometrium increased in turn, and the expression of P0.05U 路Sp1 in stromal cells and glandular epithelial cells of endometrium, mainly in the nucleus, was observed in both endometrium and glandular epithelium. The expression of Sp1 mRNA and protein in ectopic endometrium, eutopic endometrium and normal endometrium decreased in turn, and the expression of P0.05N. MiR23b was negatively correlated with Sp1 mRNA expression. Conclusion the ratio MiR23b and Sp1 are closely related to the occurrence and development of ovarian endometriosis. They may be involved in the formation of ectopic lesions through mutual antagonism.
【作者单位】：中南大学湘雅医院妇产科;
【基金】：中南大学湘雅医院青年科研基金(2014Q09)~~
【分类号】：R711.71

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