FoxO4与子痫前期蜕膜血管急性动脉粥样硬化的关系

发布时间：2018-05-01 23:10

本文选题：子痫前期 + FoxO4　；参考：《中南大学》2014年硕士论文

【摘要】：目的：通过研究FoxO4在子痫前期患者蜕膜中表达的变化,分析其在蜕膜血管急性动脉粥样硬化形成中的作用,并阐明其与母体氧化应激反应的关系,从而探讨FoxO4如何参与了子痫前期这一疾病的发生发展。方法：选取2013年7月～2014年3月在中南大学湘雅二医院确诊为“子痫前期”的25例孕妇作为研究组,重度子痫前期15例,轻度子痫前期10例；选取同期正常妊娠的15例孕妇作为对照组,两组孕妇在分娩未发动之前均以剖宫产终止妊娠。将40例患者蜕膜组织切片行HE染色,根据HE染色结果再次进行分组。采用酶联免疫吸附试验(ELISA)分别检测各组孕妇血清中8-异前列腺素蛋白浓度,并进行统计学分析；采用免疫印迹法(Western Blot)分别检测各组孕妇蜕膜中8-异前列腺素、FoxO4及磷酸化FoxO4(P-FoxO4)的蛋白表达水平,并进行统计学分析,利用免疫组化方法检测各组蜕膜中的P-FoxO4蛋白表达定位。同时进一步分析了重度子痫前期、轻度子痫前期及正常对照组中蜕膜FoxO4、P-Foxo4,血清及蜕膜中8-异前列腺素的表达差异。结果：1.子痫前期患者蜕膜中FoxO4及磷酸化FoxO4较正常对照组升高,且重度组高于轻度组；同时发现子痫前期患者血清及蜕膜中8-异前列腺素较正常对照组升高,且重度组高于轻度组。2.蜕膜HE染色显示：子痫前期患者中蜕膜血管“急“性动脉粥样硬化病变”发生率为40%,正常对照组患者蜕膜血管均无病变。3.依此将患者分为子痫前期伴有蜕膜血管“急性动脉粥样硬化”组(PE+Aa);子痫前期不伴蜕膜血管“急性动脉粥样硬化”组(PE-Aa);正常对照组(NP)。4.三组孕妇蜕膜中FoxO4蛋白相对灰度值呈下降趋势(P0.01)：PE+Aa组0.63±0.10；PE-Aa组0.48±0.07；NP组0.33±0.04。5.三组孕妇蜕膜中P-FoxO4蛋白相对灰度值呈下降趋势(P0.01)：PE+Aa组0.50±0.09：PE-Aa组0.40±0.06；NP组0.26±0.04。6.PE+Aa组孕妇蜕膜中存在大量表达P-FoxO4的蜕膜细胞,且明显多于PE-Aa组及NP组。7.三组孕妇血清中8-异前列腺素浓度呈下降趋势(P0.01)：PE+Aa组338.1±27.5pg/ml; PE-Aa组250.8±30.1pg/ml; NP组155.6±11.0pg/ml。8.三组孕妇蜕膜中8-异前列腺素蛋白相对灰度值呈下降趋势(P0.01)：PE+Aa组0.53±0.07；PE-Aa组0.41±0.09；NP组0.31±0.07。9.三组孕妇蜕膜组织中FoxO4及P-FoxO4的蛋白水平均与蜕膜组织及外周血中8-异前列腺素蛋白水平呈正相关关系。结论：1.重度子痫前期孕妇蜕膜中FoxO4、P-FoxO4及8-异前列腺素的表达均较轻度组及正常组增高。且重度子痫前期患者中,蜕膜血管“急性动脉粥样硬化”病变发生率较轻度组及正常组升高。2.在子痫前期孕妇中,有蜕膜血管“急性动脉粥样硬化”组的FoxO4, P-FoxO4及8-异前列腺素表达水平较无蜕膜血管“急性动脉粥样硬化”组升高。3.FoxO4可能通过调控蜕膜血管“急性动脉粥样硬化”的形成,参与推动了子痫前期孕妇局部及全身的氧化应激失衡。
[Abstract]:Objective: to study the expression of FoxO4 in decidua of preeclampsia patients, to analyze its role in the formation of acute atherosclerosis of decidual vessels, and to elucidate the relationship between the expression of FoxO4 and maternal oxidative stress. To explore how FoxO4 is involved in the development of preeclampsia. Methods: from July 2013 to March 2014, 25 pregnant women diagnosed as "preeclampsia" in Xiangya No.2 Hospital of Central South University were selected as study group. 15 cases were severe preeclampsia and 10 cases were mild preeclampsia. 15 pregnant women with normal pregnancy were selected as control group. The pregnant women in both groups were terminated by cesarean section before delivery. The decidua sections of 40 patients were stained by HE staining and grouped according to the results of HE staining. Serum 8-isoprostaglandin protein (8-isoprostaglandin) concentration in pregnant women in each group was detected by enzyme linked immunosorbent assay (Elisa) and analyzed statistically. The expression levels of 8-isoprostaglandin FoxO4 and phosphorylated FoxO4 P-FoxO4 in decidua of pregnant women were detected by Western blot. The expression of P-FoxO4 protein in decidua of each group was detected by immunohistochemical method. At the same time, we further analyzed the difference of the expression of 8-isoprostaglandin in the serum and decidua of severe preeclampsia, mild preeclampsia and normal control group. The result is 1: 1. The levels of FoxO4 and phosphorylated FoxO4 in decidua of preeclampsia patients were higher than those in normal controls, and the levels of 8-isoprostaglandin in serum and decidua of patients with preeclampsia were higher than those in mild eclampsia, and those in severe group were higher than those in mild group. He staining of decidua showed that the incidence of decidual vascular "acute" atherosclerosis was 40 in preeclampsia patients, while that in normal control group was not. 3. The patients were divided into preeclampsia with decidual "acute atherosclerosis" group, preeclampsia without decidual vessel "acute atherosclerosis" group and normal control group with NP.4. The relative gray value of FoxO4 protein in decidua of three groups showed a decreasing trend: P0.01: PE-Aa group 0.63 卤0.10 PE-Aa group 0.48 卤0.07 PE-Aa group 0.33 卤0.04.5 in NP group. The relative gray value of P-FoxO4 protein in decidua of the three groups showed a decreasing trend. There were a large number of decidua cells expressing P-FoxO4 in decidua of pregnant women in the group of 0.50 卤0.09:PE-Aa 0.40 卤0.06NP-0.26 卤0.04.6.PE AA, which were significantly more than those in group PE-Aa and group NP. The concentration of 8-isoprostaglandin in serum of pregnant women in the three groups showed a decreasing trend: P0.01A group (338.1 卤27.5 PG / ml); PE-Aa group (250.8 卤30.1pg / ml); NP group (155.6 卤11.0pg / ml 路8pg / ml). The relative gray value of 8-isoprostaglandin protein in decidua of the three groups showed a decreasing trend: P0.01: PEAa 0.53 卤0.07 PE-Aa 0.41 卤0.09 PE-Aa 0.41 卤0.09 NP 0.31 卤0.07.9. The protein levels of FoxO4 and P-FoxO4 in decidua tissue were positively correlated with 8-isoprostaglandin protein level in decidua tissue and peripheral blood of the three groups. Conclusion 1. The expression of FoxO4 P-FoxO4 and 8-isoprostaglandin in decidua of pregnant women with severe preeclampsia was higher than that of mild group and normal group. In patients with severe preeclampsia, the incidence of decidual vascular "acute atherosclerosis" was higher than that of mild and normal groups. Among pregnant women with preeclampsia, The expression levels of FoxO4, P-FoxO4 and 8-isoprostaglandin in decidual vessel "acute atherosclerosis" group were higher than those without decidual vessel "acute atherosclerosis" group. 3. FoxO4 may regulate the formation of decidual vascular "acute atherosclerosis". Participation promotes local and systemic oxidative stress imbalance in preeclampsia pregnant women.
【学位授予单位】：中南大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R714.244

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