端粒酶逆转录酶在子宫内膜异位症中的表达

发布时间：2018-05-02 10:13

  本文选题：子宫内膜异位症 + 端粒酶　； 参考：《山东大学》2014年硕士论文

【摘要】：研究背景及目的：子宫内膜异位症(Endometriosis, EMs)是妇科常见的难治性良性疾病之一,在生育年龄女性的发病率约为6-10%。腹腔镜仍是目前诊断子宫内膜异位症的金标准。端粒(telomere)位于真核生物染色体的末端,具有维持染色体稳定的功能,其长度对于控制细胞有丝分裂次数具有重要作用。端粒酶(telomerase)具有维持端粒长度的作用,端粒酶的活化可以使细胞克服衰老获得无限分裂的能力。端粒酶逆转录酶(human telomerase reverse transcriptase, hTERT)是端粒酶的催化亚基,对细胞内端粒酶的活性起决定性作用。研究证明,hTERT在多种肿瘤细胞中高表达,而hTERT在子宫内膜异位症中的研究较少。本文通过研究hTERT在子宫内膜异位症的在位内膜组织及异位内膜组织中的表达情况,评估hTERT在子宫内膜异位症的发病机制中可能起到的作用。 方法：应用免疫组织化学染色sP法检测54例子宫内膜异位症患者及15例正常子宫内膜细胞内hTERT的表达情况,比较hTERT在子宫内膜异位症在位内膜组织及异位内膜组织中的表达与正常子宫内膜中hTERT表达的差异,并且分析在子宫内膜异位症中hTERT表达情况与CA125、r-AFS分期及年龄之间的相关关系。 结果：子宫内膜异位症在位内膜中hTERT的表达水平比正常子宫内膜中hTERT的表达水平显著增高(p0.05)；子宫内膜异位症异位病灶处hTERT的表达水平比在位内膜中hTERT的表达水平显著增高(p0.05),并且比正常子宫内膜组织中hTERT的表达水平明显增高(p0.05)。在子宫内膜异位症在位内膜和正常内膜中hTERT的表达与内膜的周期性变化无明显差异(p0.05)。hTERT在子宫内膜异位症在位内膜在增生期的表达比正常子宫内膜增生期hTERT的表达显著增强(p0.05)。hTERT在子宫内膜异位症在位内膜在月经分泌期中的表达比正常子宫内膜分泌期hTERT的表达无明显差异(p0.05)。无论是在正常内膜还是在子宫内膜异位症的在位内膜中hTERT的表达与月经周期变化无明显统计学差异(p0.05)。子宫内膜异位症中hTERT的表达与血清中CA125水平呈正相关((p0.05,r0)；与r-AFS分期(p0.05)和年龄(p0.05)无明显相关性。 结论：hTERT在子宫内膜异位症中的异常表达说明hTERT在子宫内膜异位症的发生过程中起一定的作用,子宫内膜异位症的发生可能与在位内膜细胞及异位细胞的异常增生有关。hTERT的免疫组织化学染色联合血清CA125可对评估子宫内膜异位症的恶变有一定的意义。
[Abstract]:Background and objective: Endometriosism (EMs) is one of the common refractory benign diseases in gynecology. Laparoscopy is still the gold standard for the diagnosis of endometriosis. Telomere, which is located at the end of eukaryotic chromosome, has the function of maintaining chromosome stability, and its length plays an important role in controlling the number of cell mitosis. Telomerase has the function of maintaining telomere length. The activation of telomerase can make cells overcome aging and gain infinite division ability. Human telomerase reverse transcriptase (hTERT) is a catalytic subunit of telomerase, which plays a decisive role in telomerase activity. It has been demonstrated that hTERT is highly expressed in various tumor cells, while hTERT is rarely studied in endometriosis. By studying the expression of hTERT in eutopic endometrium and ectopic endometrium of endometriosis, we evaluated the role of hTERT in the pathogenesis of endometriosis. Methods: the expression of hTERT in 54 cases of endometriosis and 15 cases of normal endometrium were detected by immunohistochemical staining SP method. To compare the expression of hTERT in eutopic endometrium and ectopic endometrium and hTERT expression in normal endometrium, and to analyze the correlation between hTERT expression and CA125 r-AFS stage and age in endometriosis. Results: the expression level of hTERT in eutopic endometrium in endometriosis was significantly higher than that in normal endometrium, and the expression level of hTERT in ectopic endometriosis was higher than that in eutopic endometrium. The expression of hTERT in endometrium was significantly higher than that in normal endometrium. The expression of hTERT in eutopic endometrium and normal endometrium is not significantly different from the periodic change of endometrium. The expression of p0.05 hTERT in eutopic endometrium is higher than that in normal endometrium in proliferative phase. The expression of p0.05 hTERT in eutopic endometrium in endometriosis during menstrual secretion was significantly enhanced compared with that in normal endometrium. There was no significant difference in the expression of p0.05T between eutopic endometrium and normal endometrium. There was no significant difference between the expression of hTERT and the change of menstrual cycle in normal endometrium and eutopic endometrium. The expression of hTERT in endometriosis was positively correlated with the level of CA125 in serum, but not with r-AFS staging (p0.05) and age (p0.05). Conclusion the abnormal expression of hTERT in endometriosis suggests that hTERT may play a role in the pathogenesis of endometriosis. The occurrence of endometriosis may be related to the abnormal proliferation of eutopic endometrial cells and ectopic cells. The immunohistochemical staining of hTERT combined with serum CA125 may be useful in evaluating the malignant change of endometriosis.
【学位授予单位】：山东大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R711.71

【参考文献】

相关期刊论文 前2条

1 屈振亮;端粒酶逆转录酶基因的结构特点及转录调控[J];国外医学(分子生物学分册);2002年05期

2 郎景和;子宫内膜异位症研究的新里程[J];中华妇产科杂志;2005年01期

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本文编号：1833465