饮食干预对妊娠期糖尿病小鼠子代中远期瘦素及糖脂代谢的影响

发布时间：2018-05-04 19:32

本文选题：妊娠期糖尿病 + 瘦素　；参考：《现代预防医学》2016年15期

【摘要】：目的研究妊娠期糖尿病(GDM)子代小鼠在饮食干预下其瘦素水平及糖脂代谢的转归情况。方法链脲霉素腹腔注射建立GDM模型,将其产下子鼠随机分为对照组、高脂组(G-HF)、高纤组(G-HX)、热量限制组(G-CR),非GDM子鼠建立空白对照组(C-CON);每组各7只。干预后测总胆固醇(TCH),甘油三酯(TG),低密度脂蛋白胆固醇(LDL-C),高密度脂蛋白胆固醇(HDL-C),胰岛素、瘦素水平。结果 (1)GDM子代血糖显著高于正常鼠子代(P0.05);TCH,TG,LDL-C显著升高(P0.05)。(2)饮食干预后,热量限制组血糖体重趋于稳定;高脂组血脂代谢水平均显著降低(P0.05);高纤组HDL-C高于空白对照组(P0.05);热量限制组TCH,TG,LDL-C明显降低(P0.001),瘦素、胰岛素改善。(3)血清瘦素水平与TG、TCH、LDL-C及胰岛素水平正相关(P0.05)。结论 GDM子鼠有明显的不良结局。高脂饮食能加重GDM子代不良转归,高纤饮食作用较弱,热量限制可能通过改善瘦素抵抗,显著改善胰岛素抵抗及糖脂代谢。
[Abstract]:Objective to study the level of leptin and the outcome of glucose and lipid metabolism in GDM offspring of gestational diabetes mellitus (GDM). Methods the GDM model was established by intraperitoneal injection of streptozotocin. The mice were randomly divided into control group, high fat group, high fiber group, caloric restriction group and non-GDM control group, with 7 rats in each group. After intervention, the levels of total cholesterol (TCHG), triglyceride (TG), low density lipoprotein cholesterol (LDL-C), high density lipoprotein cholesterol (HDL-C), insulin and leptin were measured. Results the blood glucose of the GDM offspring was significantly higher than that of the normal offspring (P0.05TGN LDL-C increased significantly (P0.05U. 2)) after dietary intervention, the blood glucose and body weight of the caloric restricted group tended to be stable. In the hyperlipidemia group, the serum lipid metabolism level was significantly decreased, the HDL-C level in the high fiber group was higher than that in the blank control group (P 0.05), and the LDL-C level in the caloric restriction group was significantly lower than that in the control group (P 0.001, leptin, insulin improvement.) the serum leptin level was positively correlated with the levels of TGG TCHN LDL-C and insulin in the hyperlipidemia group (P 0.05). Conclusion GDM mice have obvious adverse outcomes. High-fat diet can aggravate the adverse outcome of GDM offspring, but the effect of high-fiber diet is weak. Caloric restriction may improve insulin resistance and glycolipid metabolism by improving leptin resistance.
【作者单位】：安徽医科大学公共卫生学院营养与食品卫生学系;
【基金】：国家自然科学基金(81373011)
【分类号】：R714.256;R459.3

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