子宫内膜癌组织中miR-126、EGFL7表达及意义

发布时间：2018-05-05 01:30

本文选题：子宫内膜癌 + 微小RNA-　；参考：《山东医药》2015年40期

【摘要】：目的探讨微小RNA-126(miR-126)、类表皮生长因子域7(EGFL7)在子宫内膜癌发生、发展中的作用。方法选择子宫内膜癌患者57例、子宫内膜增生症患者43例,分别取子宫内膜癌组织(内膜癌组)、癌旁正常组织(癌旁组)及子宫内膜增生组织(增生组)。采用qRT-PCR法检测各组miR-126、EGFL7 RNA相对表达量,免疫组化SP法检测微血管密度(MVD)。结果内膜癌组miR-126 RNA相对表达量低于增生组、癌旁组,增生组低于癌旁组(P均0.05);内膜癌组EGFL7 RNA相对表达量、MVD均高于增生组、癌旁组,增生组高于癌旁组(P均0.05)。Pearson相关分析显示,内膜癌组miR-126 RNA相对表达量与EGFL7 RNA相对表达量、MVD均呈负相关(r分别为-0.507、-0.873,P均0.05),EGFL7 RNA相对表达量与MVD呈正相关(r=0.416,P0.05)。内膜癌组miR-126、EGFL7 RNA相对表达量均与患者FIGO分期、淋巴结转移有关(P均0.05)。结论子宫内膜癌组织中miR-126呈低表达、EGFL7呈高表达;二者可能共同参与了子宫内膜癌的发生、发展及侵袭、转移过程。
[Abstract]:Objective to investigate the role of minimal RNA-126 miR-126 and EGFL7 in the development of endometrial carcinoma. Methods 57 cases of endometrial carcinoma and 43 cases of endometrial hyperplasia were selected. The tissues of endometrial carcinoma (endometrial carcinoma group), adjacent normal tissue (paracancerous group) and endometrial hyperplasia tissue (proliferative group) were collected respectively. The relative expression of miR-126 EGFL7 RNA was detected by qRT-PCR method, and the microvessel density (MVD) was detected by immunohistochemical SP method. Results the relative expression of miR-126 RNA in the endometrial carcinoma group was lower than that in the proliferative group, the relative expression of EGFL7 RNA in the paracancerous group and the proliferative group was lower than that in the paracancerous group (P < 0.05), and the relative expression of EGFL7 RNA in the endometrial carcinoma group was higher than that in the proliferative group. There was a negative correlation between the relative expression of miR-126 RNA and the relative expression of EGFL7 RNA in endometrial carcinoma group. The relative expression of EGFL7 RNA was positively correlated with that of MVD. The relative expression of miR-126 EGFL7 RNA in endometrial carcinoma was correlated with FIGO stage and lymph node metastasis (P < 0.05). Conclusion low expression of miR-126 and high expression of EGFL7 in endometrial carcinoma may be involved in the occurrence, development, invasion and metastasis of endometrial carcinoma.
【作者单位】：新乡市中心医院;
【分类号】：R737.33

【参考文献】