不同宫颈处理方法对宫颈管分泌物细菌培养结果的影响

发布时间：2018-05-06 21:34

本文选题：宫颈管分泌物 + 细菌培养　；参考：《山西医科大学》2014年硕士论文

【摘要】：目的比较宫颈不同处理方法对宫颈管分泌物细菌培养结果的影响。方法将细菌性阴道病、阴道清洁度III-IV度的盆腔炎性疾病患者80例,按照宫颈外口处理方法不同分为五组。A组：用无菌拭子经宫颈外口进入宫颈管取分泌物；B组：无菌干棉球擦拭宫颈外口后取材；C组：生理盐水、无菌干棉球先后擦拭宫颈外口后取材；D组：0.5%碘伏擦拭宫颈外口后取材；E组：0.5%碘伏、生理盐水、无菌干棉球依次擦拭宫颈外口后取材。每组均同时取阴道分泌物行细菌培养。根据菌落的不同,挑取不同菌落行革兰氏染色,进行菌种的识别,计数培养基中菌种数。对不同方法处理宫颈外口后宫颈管细菌培养菌种数比较,配对比较宫颈管与阴道分泌物细菌培养菌种数。结果A组与阴道分泌物细菌培养菌种数比较差异无统计学意义(5.31±1.62,6.06±0.68,P0.05)；B组与阴道分泌物细菌培养菌种数比较差异有统计学意义(3.88±1.09,6.19±0.66,P0.001)；C组与阴道分泌物细菌培养菌种数比较差异有统计学意义(3.38±1.20,6.06±0.57,P0.001)；D组与阴道分泌物细菌培养菌种数比较差异有统计学意义(0.69±0.48,6.06±0.44,P0.001);E组与阴道分泌物细菌培养菌种数比较差异有统计学意义(3.25±0.45,6.13±0.62,P0.001)。五组阴道分泌物细菌培养菌种数差异无统计学意义(F=0.139,P=0.967)。A组与其它四组宫颈管分泌物细菌培养菌种数比较差异均有统计学意义(P0.05)；B组与C组宫颈管分泌物细菌培养菌种数比较差异无统计学意义(P0.05),与D组比较差异有统计学意义(P0.05),与E组比较差异无统计学意义(P0.05)；C组与D组宫颈管分泌物细菌培养菌种数比较差异有统计学意义(P0.05),与E组比较差异无统计学意义(P0.05)；D组与E组宫颈管分泌物细菌培养菌种数比较差异有统计学意义(P0.05)。结论取宫颈管分泌物细菌培养前有必要对宫颈外口进行预处理；对宫颈外口进行简单的处理如无菌干棉球擦拭,就可以降低阴道微生物的污染,同时简化了取材前宫颈外口处理方法。
[Abstract]:Objective to compare the effect of different treatment methods on bacterial culture of cervical secretion. Methods 80 patients with bacterial vaginosis and pelvic inflammatory disease with III-IV degree of vaginal cleanliness were studied. According to the different methods of external cervix treatment, group A was divided into five groups: group B: sterilized swab was used to enter the cervix canal through cervix to take secretion from cervical canal, group B: group C: normal saline after wiping cervix external mouth with aseptic dry cotton ball. Sterile dry cotton balls were used to wipe the external cervix of cervix. Group D: 0.5% iodophor was taken after wiping cervical external mouth. Group E: 0.5% iodophor, physiological saline, sterile dry cotton ball were used to wipe the material after cervical external mouth in turn. Vaginal secretions were taken from each group at the same time for bacterial culture. According to the different colonies, different colonies were picked up for Gram staining to identify the species and count the number of strains in the culture medium. The number of bacteria cultured in the cervical canal treated with different methods was compared, and the number of bacteria cultured in the cervical canal and vaginal secretion was compared. Results there was no significant difference between group A and group A in the number of bacteria cultured in vaginal secretions. There was no significant difference between group A and group B in the number of bacteria cultured in vaginal secretions. There was a significant difference between group B and group B in the number of bacteria cultured in vaginal secretions. There was a significant difference between group C and group C in the number of bacteria cultured in vaginal secretions. There was significant difference between group D (3.38 卤1.206.06 卤0.57p 0.001) and vaginal secretion (P 0.001). There was significant difference between group E and group E (0.69 卤0.48U, 6.06 卤0.44P 0.001) and group E (3.25 卤0.456.13 卤0.62P 0.001). There was no significant difference in the number of bacteria cultured in vaginal secretions among the five groups. There were significant differences between group A and the other four groups in the number of bacteria cultured in cervical secretions. There were significant differences in the number of bacteria cultured in cervical secretions between group A and group C. there were significant differences between group A and group C in the number of bacteria cultured in cervical secretions. There was no significant difference in the number of bacteria in cervical secretion between group C and group D, but there was no significant difference between group D and group D, and there was no significant difference between group E and group C in the number of bacteria cultured in cervical canal secretion. There was a significant difference between group C and group D in the number of bacteria cultured in cervical canal secretion. There was no significant difference between group D and group E in the number of bacteria cultured in the secretion of cervical canal. There was significant difference between group D and group E in the number of bacteria cultured in the secretion of cervical canal (P0.05). Conclusion it is necessary to pretreat the external cervix orifice before the bacterial culture of the cervical canal secretions, and the simple treatment of the external cervix orifice, such as wiping the sterile dry cotton ball, can reduce the contamination of the vaginal microorganism. At the same time, it simplifies the treatment method of cervical external orifice before sampling.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R711.3

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