鼠尾草酚抑制卵巢癌生长及其机制研究

发布时间：2018-05-08 22:09

本文选题：鼠尾草酚 + 卵巢癌　；参考：《中华中医药学刊》2015年12期

【摘要】：目的:研究鼠尾草酚对卵巢癌生长的影响及其机制。方法:不同浓度的鼠尾草酚(2.5μmol·L~(-1)、5μmol·L~(-1)、10μmol·L~(-1))作用卵巢癌细胞株SKOV3 24 h后,CCK-8法检测细胞增殖;流式细胞术检测细胞凋亡;Western blot检测SKOV3细胞中PI3K、P-Akt、FOXO3a(Forkhead box O3,FOXO3a)、X连锁凋亡抑制蛋白(X-linked inhibitor of apoptosis protein,XIAP)、Akt蛋白的表达。建立起裸鼠卵巢癌皮下移植瘤模型,观察不同浓度的鼠尾草酚对卵巢癌皮下移植瘤生长的影响;免疫组织化学法检测肿瘤组织中FOXO3a、XIAP阳性表达。结果:与对照组相比,鼠尾草酚可明显抑制卵巢癌SKOV3细胞生长,并显著增加癌细胞凋亡率,呈浓度依赖性;鼠尾草酚可下调PI3K、P-Akt、XIAP蛋白表达,同时上调FOXO3a。鼠尾草酚各剂量组卵巢癌组织中XIAP阳性表达率均低于对照组,而FOXO3a阳性表达率均高于对照组。结论:鼠尾草酚可抑制卵巢癌细胞的生长,其机制可能通过PI3K/Akt信号通路,诱导其凋亡。
[Abstract]:Objective: to study the effect of sage on the growth of ovarian cancer and its mechanism. Methods: the proliferation of ovarian cancer cell line SKOV3 was detected by CCK-8 method 24 h after treated with different concentrations of sage 2.5 渭 mol L ~ (1) and 5 渭 mol / L ~ (5 渭 mol / L), and the expression of PI3KP-AktFOXO _ (3) -Forkhead box O3FXO _ (3a) X linked apoptosis inhibitor protein X-linked inhibitor of apoptosis protein (XIAP-Akt) in SKOV3 cells was detected by flow cytometry (FCM) and flow cytometry (FCM) respectively, and the expression of PI3KP-AktFOXO3a (Forkhead box O3FXO3FX) -linked apoptosis inhibitor protein X-linked inhibitor of apoptosis protein (XIAP-Akt) was detected by flow cytometry. The subcutaneous transplanted tumor model of ovarian cancer in nude mice was established to observe the effect of different concentrations of sage on the growth of ovarian cancer subcutaneous transplanted tumor and the positive expression of FOXO3a XIAP in tumor tissue was detected by immunohistochemical method. Results: compared with the control group, sage can significantly inhibit the growth of ovarian cancer SKOV3 cells and increase the apoptosis rate in a dose-dependent manner, and sage phenol can down-regulate the expression of PI3KP- AktOXIAP and up-regulate FOXO3a.Results: compared with the control group, sage can significantly inhibit the growth of ovarian cancer SKOV3 cells and increase the apoptosis rate in a dose-dependent manner. The positive expression rate of XIAP and FOXO3a in ovarian cancer tissues of each dose of sage was lower than that of control group, but the positive rate of FOXO3a was higher than that of control group. Conclusion: sage can inhibit the growth of ovarian cancer cells, which may be induced by PI3K/Akt signaling pathway.
【作者单位】：温州医科大学附属第一医院妇产科;
【基金】：国家自然科学基金项目(81070510)
【分类号】：R737.31

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