子痫前期患者血清中ABCA1的表达及其对内皮细胞损伤的影响

发布时间：2018-05-09 19:32

本文选题：子痫前期 + 三磷酸腺苷结合盒转运体A　；参考：《吉林大学学报(医学版)》2015年03期

【摘要】：目的:探讨子痫前期患者外周静脉血清中三磷酸腺苷结合盒转运体A1(ABCA1)蛋白表达的特点,阐明ABCA1对内皮细胞损伤的影响及可能的机制。方法:选取同期住院、年龄、孕周无明显差异的子痫前期患者40例(按病情轻重分为轻度、重度组)及正常妊娠者20名,采用ELISA试剂盒定量检测3组研究对象外周静脉血清中ABCA1及氧化低密度脂蛋白(OX-LDL)表达水平的差异。原代分离并培养上述3组研究对象脐静脉内皮细胞(HUVECs),采用荧光标记的牛血清白蛋白(BSA)检测内皮细胞的单层屏障功能;采用MTT法检测内皮细胞的增殖能力;采用硝酸还原酶法检测内皮细胞的一氧化氮(NO)合成能力;计算OX-LDL/ABCA1比值,并进行相关性分析。结果:与正常妊娠组比较,子痫前期组患者外周静脉血清中ABCA1蛋白表达水平明显降低(P0.05),而OX-LDL表达水平及OX-LDL/ABCA1比值明显升高(P0.05);ABCA1与OX-LDL表达水平呈明显负相关关系(r=-0.681,P0.05)。与正常妊娠组比较,子痫前期组患者通过单层内皮细胞的BSA水平明显升高(P0.05),内皮细胞增殖能力明显下降(P0.05);内皮细胞分泌NO水平明显降低(P0.05)。且病情越严重,上述改变越明显。子痫前期组患者血清中OX-LDL/ABCA1比值与通过内皮细胞的BSA水平呈正相关关系(r=0.791,P0.05),与内皮细胞的增殖水平呈负相关关系(r=-0.812,P0.05),与内皮细胞中NO水平也呈负相关关系(r=-0.635,P0.05)。结论:子痫前期患者血清中ABCA1蛋白表达水平明显下降,引起氧化应激,OX-LDL和ABCA1表达失衡,这与全身血管内皮细胞的损伤密切相关,可能是参与子痫前期发生发展的重要机制之一。
[Abstract]:Aim: to investigate the expression of adenosine triphosphate binding cassette transporter A1 (A1) ABCA1 protein in peripheral venous serum of patients with preeclampsia and to elucidate the effect of ABCA1 on endothelial cell injury and its possible mechanism. Methods: 40 patients with preeclampsia who were hospitalized, age and gestational week were divided into mild group and severe group according to the severity of the disease and 20 normal pregnant women. The expression of ABCA1 and oxidized low density lipoprotein (OX-LDL) in peripheral vein serum of the three groups were quantitatively detected by ELISA kit. The umbilical vein endothelial cells (HUVECs) were isolated and cultured in primary culture, the monolayer barrier function of endothelial cells was detected by fluorescence labeled bovine serum albumin (BSA), and the proliferation of endothelial cells was detected by MTT method. The nitric oxide (no) synthesis of endothelial cells was measured by nitrate reductase method, and the ratio of OX-LDL/ABCA1 was calculated and the correlation analysis was carried out. Results: compared with the normal pregnancy group, the expression of ABCA1 protein in the peripheral vein of preeclampsia group was significantly lower than that in the normal pregnancy group, while the expression of OX-LDL and the ratio of OX-LDL/ABCA1 were significantly increased in the preeclampsia group. There was a negative correlation between the expression level of ABCA1 and the expression level of OX-LDL in the preeclampsia group. Compared with the normal pregnancy group, the BSA level of the monolayer endothelial cells in the preeclampsia group was significantly higher than that in the normal pregnancy group, and the proliferation ability of the endothelial cells was decreased significantly (P 0.05), while the level of no secreted by the endothelial cells decreased significantly (P 0.05). The more serious the disease, the more obvious the above changes were. In preeclampsia group, the ratio of OX-LDL/ABCA1 in serum was positively correlated with the level of BSA passing through endothelial cells, negatively correlated with the level of proliferation of endothelial cells, and negatively correlated with the level of no in endothelial cells. Conclusion: the expression of ABCA1 protein in serum of patients with preeclampsia is obviously decreased, which leads to the imbalance of OX-LDL and ABCA1 expression in patients with preeclampsia, which is closely related to the injury of systemic vascular endothelial cells and may be one of the important mechanisms involved in the development of preeclampsia.
【作者单位】：莆田学院基础医学院人体解剖学教研室;解放军南京军区福州总医院第一附属医院妇产科;福建医科大学附属第一医院眼科;
【基金】：国家自然科学基金资助课题(81270976) 福建省教育厅科研基金资助课题(JK2013045)
【分类号】：R714.244

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