热休克蛋白70的表达与自发性早产合并组织学绒毛膜羊膜炎的关系

发布时间：2018-05-09 23:05

本文选题：早产 + 热休克蛋白　；参考：《实用妇产科杂志》2017年04期

【摘要】：目的:探讨热休克蛋白70(HSP70)的表达与自发性早产合并组织学绒毛膜羊膜炎(HCA)的关系。方法:选择2015年6月至2016年1月在郑州大学第三附属医院产科住院并分娩的早产临产孕妇46例为早产临产组,并随机选择同期足月正常分娩的孕妇31例(足月正常组)为对照组。早产临产组孕妇根据分娩后胎膜组织病理学检查结果,分为早产合并HCA组(21例)及早产未合并HCA组(25例)。ELISA法测定并比较各组孕妇外周血、脐血中HSP70蛋白水平,采用免疫组化SP法、Western blotting法及RT-PCR法检测并比较各组孕妇胎膜组织中HSP70蛋白及mRNA的表达水平。采用Pearson相关分析法分析各组HSP70蛋白的相关性。结果:(1)早产合并HCA组孕妇外周血、脐血及胎膜组织中HSP70蛋白的表达和胎膜组织中HSP70 mRNA的表达高于早产未合并HCA组,早产临产组均高于足月正常组,3组间两两比较,差异均有统计学意义(P0.01)。(2)早产合并HCA组中孕妇外周血与脐血及胎膜组织中HSP70蛋白的表达均呈正相关(P0.05)。(3)早产合并HCA组中新生儿败血症发生率高于其余两组(P0.05)。结论:HSP70在早产及其合并HCA时表达增加,HSP70可能参与了早产的发生机制,同时与HCA及新生儿败血症有关。
[Abstract]:Aim: to investigate the relationship between the expression of heat shock protein 70 (HSP70) and the expression of HCA in spontaneous preterm labor complicated with histopathological chorioamnionitis. Methods: from June 2015 to January 2016, 46 pregnant women who were hospitalized and delivered in obstetrics department of the third affiliated Hospital of Zhengzhou University were selected as preterm labor group. 31 pregnant women (term normal group) were randomly selected as control group. According to the results of histopathological examination of fetal membranes after delivery, pregnant women in premature delivery group were divided into preterm labor with HCA group (n = 21) and preterm labor with HCA group (n = 25). Elisa was used to detect and compare the levels of HSP70 protein in peripheral blood and umbilical cord blood of pregnant women in each group. The expression of HSP70 protein and mRNA in the fetal membranes of pregnant women were detected and compared by immunohistochemical SP blotting and RT-PCR methods. The correlation of HSP70 protein in each group was analyzed by Pearson correlation analysis. Results (1) the expression of HSP70 protein in peripheral blood, umbilical cord blood and fetal membrane tissue and the expression of HSP70 mRNA in fetal membrane tissue in premature labor with HCA group were higher than those in preterm labor without HCA group, and those in premature labor group were higher than those in term normal group. There was a positive correlation between the expression of HSP70 protein in peripheral blood and umbilical cord blood and fetal membrane tissue in HCA group. The incidence of neonatal septicemia in premature labor with HCA group was higher than that in other two groups (P0.05. 0. 05%, P 0. 05%, P 0. 05%, P 0. 05%, P 0. 05%, P 0. 05%, P 0. 05%, P 0. 05%, P 0. 05%). Conclusion the increased expression of HSP70 in premature delivery and its association with HCA may be involved in the pathogenesis of premature delivery, and may be related to HCA and neonatal septicemia.
【作者单位】：郑州大学第三附属医院;
【基金】：河南省卫生厅基金项目(编号:201403099)
【分类号】：R714.21

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