Dyrk1B在上皮性卵巢癌组织和细胞中的表达及意义

发布时间：2018-05-11 10:07

本文选题：卵巢癌 + DyrkB　；参考：《现代妇产科进展》2015年12期

【摘要】：目的:检测Dyrk1B在上皮性卵巢癌组织和细胞中的表达,探讨其在卵巢癌发生和发展中的作用及意义。方法:Western blot法检测卵巢癌细胞系(OVCAR8,OVCAR5,OVCAR4,OVCAR3,SKOV3,OV2008,C13,A2780S和A2780CP)中Dyrk1B蛋白表达,以及Dyrk1B抑制剂AZ191对卵巢癌细胞Dyrk1B蛋白表达的影响。MTT比色法和流式细胞术检测AZ191对卵巢癌细胞增殖能力和细胞凋亡的影响。选取我院近7年收治的上皮性卵巢癌患者119例,免疫组化SP法检测Dyrk1B蛋白在卵巢癌组织中的表达。结果:Dyrk1B蛋白在多种卵巢癌细胞系中普遍表达,AZ191可明显降低其在卵巢癌细胞中的表达;AZ191对各种卵巢癌细胞系的增殖能力均产生浓度依赖性的抑制作用(P0.05),并可诱发细胞凋亡,其凋亡率均随抑制剂浓度的增加而增高;Dyrk1B在卵巢癌组织中亦呈高表达,表达率为90.8%。结论:Dyrk1B在上皮性卵巢癌组织和细胞中均有表达,Dyrk1B抑制剂AZ191下调卵巢癌细胞Dyrk1B蛋白表达的同时,明显抑制细胞的增殖和诱发细胞凋亡。提示Dyrk1B可能在卵巢癌的发生和发展中起关键作用,有望成为临床分子靶向治疗卵巢癌的新靶点。
[Abstract]:Aim: to detect the expression of Dyrk1B in epithelial ovarian carcinoma tissues and cells and to explore its role and significance in the genesis and development of ovarian cancer. Methods the expression of Dyrk1B protein in OVCAR8 OVCAR5 and OVCAR3OVCAR3SKOV3OV2008 OVCAR3SKOV3OV2008 and the effect of Dyrk1B inhibitor AZ191 on the expression of Dyrk1B protein in ovarian cancer cell line were detected by blot assay. MTT colorimetric assay and flow cytometry were used to detect the effect of AZ191 on the proliferation and apoptosis of ovarian cancer cells. The expression of Dyrk1B protein in ovarian cancer tissues was detected by immunohistochemical SP method in 119 patients with epithelial ovarian cancer treated in our hospital in recent 7 years. Results the universal expression of the protein of 1% Dyrk1B protein in various ovarian cancer cell lines could significantly decrease the expression of AZ191 in ovarian cancer cell line. AZ191 had a concentration-dependent inhibitory effect on the proliferation of various ovarian cancer cell lines and could induce apoptosis. The apoptotic rate increased with the increase of inhibitor concentration, and the expression rate of Dyrk1B was 90.8%. Conclusion the expression of Dy rk1B in epithelial ovarian carcinoma tissue and cell line AZ191 down-regulated the expression of Dyrk1B protein, and significantly inhibited the proliferation and induced apoptosis of ovarian cancer cells. The results suggest that Dyrk1B may play a key role in the occurrence and development of ovarian cancer and may be a new molecular target for the treatment of ovarian cancer.
【作者单位】：大连医科大学附属第一医院妇产科;大连医科大学附属第一医院病理科;
【基金】：国家自然科学基金项目(No:81172457)
【分类号】：R737.31

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