活性氧调控谷胱甘肽水平对卵巢上皮性癌细胞化疗敏感性的影响

发布时间：2018-05-11 21:22

本文选题：卵巢上皮性癌 + 谷胱甘肽　；参考：《实用妇产科杂志》2015年12期

【摘要】：目的:探讨活性氧(ROS)调控谷胱甘肽(GSH)水平与卵巢上皮性癌细胞化疗敏感性的关系。方法:分别采用顺铂(DDP)、大黄素(Emo)、DDP+Emo、磷酸盐缓冲液(PBS)处理(对照组)卵巢上皮性癌DDP耐药株COC1/DDP细胞及其裸鼠荷瘤模型,然后利用MTT法检测细胞活力;记录肿瘤生长情况;应用谷胱甘肽过氧化物酶检测试剂盒检测GSH水平;并应用荧光染色法检测移植瘤ROS水平。结果:1裸鼠成瘤分组后成瘤体积、重量分别为DDP组2046.70±43.08 mm3、2.20±0.59 g,Emo组2934.24±548.76 mm~3、3.34±0.49 g,DDP+Emo组1595.60±406.73 mm~3、1.34±0.30 g,对照组2803.79±162.50 mm3、3.36±0.12 g,DDP+Emo组体积和重量均小于各组,差异有统计学意义(P0.05)。2DDP+Emo组相对于DDP组肿瘤细胞活力有明显降低(P0.05),并且该作用可以被ROS清除剂(NAC)逆转,DDP+Emo组肿瘤细胞活力明显低于DDP+Emo+NAC组(P0.05)。3荧光染色法测试组织和细胞ROS水平发现平均光密度(OD),DDP+Emo组与DDP组和DDP+Emo+NAC组相比ROS水平增高,差异有统计学意义(P0.05)。4测试裸鼠瘤体组织和细胞GSH水平发现DDP+Emo组水平均显著低于其他各组(P0.05)。5应用关联性分析发现肿瘤模型瘤体组织中各组的GSH水平与ROS水平均呈负相关(P0.05)。结论:ROS水平升高能够引起GSH水平下降,从而增加卵巢上皮性癌细胞的化疗敏感性。
[Abstract]:Aim: to investigate the relationship between the level of glutathione (GSH) and the chemosensitivity of epithelial ovarian cancer cells regulated by reactive oxygen species (Ros). Methods: the COC1/DDP cells of ovarian epithelial carcinoma (control group) were treated with cisplatin DDP, emodin and emodin respectively, and the tumor bearing model of nude mice was established. Then, the cell viability was detected by MTT method, and the tumor growth was recorded. GSH level was detected by glutathione peroxidase assay kit and ROS level was detected by fluorescence staining. Results the tumorigenic volume of DDP group was 2046.70 卤43.08 mm ~ 32.20 卤0.59 mm ~ (-1) respectively. The volume and weight of DDP group (1595.60 卤406.73 mm ~ 31.34 卤0.30 g) and control group (2803.79 卤162.50 mm ~ 33.36 卤0.12 g ~ (-1) Emo) were lower than those of each group. The difference was statistically significant (P 0.05). 2DDP Emo group was significantly lower than DDP group in tumor cell viability, and this effect could be reversed by ROS scavenger (ROS). The activity of tumor cell in DDP Emo group was significantly lower than that in DDP Emo NAC group by P0.05.3 fluorescence staining. And the level of cell ROS showed that the average optical density of DDP Emo group was higher than that of DDP group and DDP Emo NAC group. The difference was statistically significant (P 0.05). The level of GSH in tumor tissue and cell of nude mice was significantly lower in DDP Emo group than that in other groups (P0.05.5). The correlation analysis showed that the GSH level and ROS level of each group were negatively correlated with ROS level in tumor model. Conclusion the increase of Ros level can induce the decrease of GSH level and increase the chemosensitivity of epithelial ovarian cancer cells.
【作者单位】：上海市普陀区妇婴保健院;上海交通大学医学院附属仁济医院;
【基金】：上海市科委科研项目(编号:11ZR1421900)
【分类号】：R737.31

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