子宫颈上皮内病变组织中人乳头瘤病毒载量与病变程度的关系

发布时间：2018-05-13 04:26

本文选题：人乳头瘤病毒 + 病毒载量　；参考：《中国妇产科临床杂志》2017年04期

【摘要】：目的通过检测各级别宫颈上皮内病变组织中不同亚型HPV病毒载量,探讨HPV型别病毒载量与宫颈癌发生的相关关系。方法选择病理诊断明确的CIN1、CIN2、CIN3与宫颈癌患者各20例,采用微切技术获取宫颈病变组织,以实时荧光PCR技术检测不同病变单位细胞(10 000个细胞)内的HPV亚型病毒载量。结果(1)宫颈病灶中HPV亚型感染率在CIN1、CIN2/3与宫颈癌分别为88%、100%和100%。HPV亚型在不同级别病变的分布不同,HPV16亚型在CIN3/宫颈癌的感染率(85%)明显高于CIN2(46%)与CIN1(47%),差异有统计学意义(P0.05);HPV52亚型在CIN1、2、3中检出频率相当;HPV58亚型则在低度病变检出频率为高。(2)单位病变组织内HPV载量随病变程度增加而升高(P0.05),感染率占前三位的HPV16、52、58亚型单位病变组织内的病毒载量在CIN2/3明显高于CIN1(P0.05)。(3)HPV多重感染在CIN1、2、3与宫颈癌分别为47.1%、21.4%%、50%和26.7%,多重感染与病变的严重程度无相关性(r=-0.106,P0.05)。结论宫颈病灶组织中HPV16、52、58亚型与宫颈病变密切相关,病变单位细胞内的HPV病毒载量随宫颈病变程度增加而升高。
[Abstract]:Objective to investigate the relationship between HPV type viral load and cervical carcinogenesis by detecting the viral load of different subtypes of HPV virus in cervical intraepithelial lesions. Methods Twenty patients with CIN1-CIN2CIN3 and 20 patients with cervical cancer were selected. The tissue of cervix was obtained by microdissection, and the HPV subtype viral load was detected by real-time fluorescence PCR technique in 10 000 cells of different pathological units. Results 1) the infection rate of HPV subtype in cervical lesions was 88.00% in CIN1CIN2 / 3 and 88.00% in cervical carcinoma and different distribution of 100%.HPV subtype in different grade lesions. The infection rate of HPV16 subtype in cervical carcinoma of CIN3/ was 85% higher than that of CIN2P1-46) and CIN1CIN47.The difference was statistically significant (P0.05). The detection frequency of HPV58 subtype in CIN1O2HPV3 was higher than that of HPV58 subtype in low-grade lesions.) the HPV load increased with the increase of lesion degree. The viral load of HPV16 / 52N58 subtype of HPV16 was the highest in the first three cases. In CIN2/3, 47.1% and 26.7% in CIN1 + 2T3 and cervical cancer, respectively, there was no correlation between multiple infection and severity of lesion. There was no correlation between multiple infection and severity of lesion (P 0.05). Conclusion HPV16 / 52P58 subtype in cervical lesions is closely related to cervical lesions, and the HPV viral load in the cells of lesion units increases with the increase of cervical lesions.
【作者单位】：北京大学深圳医院妇产科深圳市妇产重大疾病早期诊断技术重点实验室;
【基金】：基金项目:深圳市科创委(GCZX2015043016200372)
【分类号】：R737.33

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