GSTP1和GSTM1基因多态性与卵巢浆液性腺癌患者生存的相关性分析

发布时间：2018-05-13 05:01

本文选题：单核苷酸多态性 + 化学治疗　；参考：《中华肿瘤防治杂志》2015年17期

【摘要】：目的探讨谷胱甘肽S-转移酶P1(glutathione S-transferase P1,GSTP1)和谷胱甘肽S-转移酶M1(glutathione S-transferase M1,GSTM1)基因单核苷酸多态性对化疗治疗卵巢浆液性囊腺癌患者生存的影响。方法选取2004-08-31-2012-08-01就诊临沂市人民医院妇科的106例患者,应用紫杉醇联合卡铂(taxol+carboplatin,TP)方案64例,其他化疗方案(拓扑替康、环磷酰胺、异环磷酰胺或吉西他滨)42例。应用焦磷酸测序法检测GSTP1和GSTM1的单核苷酸基因多态性(GSTP1Ile105Val,GSTM1缺失型/GSTM1非缺失型)。基因型和总体生存率之间的关联使用Kaplan-Meier生存曲线和Cox比例风险回归模型进行评估。结果总生存率(overall survival,OS)比较结果显示,TP组患者总生存率为38.1%,显著高于其他化疗组的23.2%,Plog-rank=0.006。GSTP1(异亮氨酸纯合子,A/A)基因型患者总生存率为34.6%,GSTP1(异亮氨酸杂合子,A/G)总生存率为57.6%,差异无统计学意义,χ2=0.132,Plog-rank=0.716。Cox比例风险回归分析结果显示,GSTP1(A/G)与等位基因(A/A)风险系数比为1.20,95%CI为0.55~2.63,P=0.65。未检测到GSTP1缬氨酸等位基因纯合子载体(G/G)。GSTM1缺失型患者总生存率为29.5%,GSTM1非缺失型患者总生存率为74.9%,差异无统计学意义,χ2=0.015,Plog-rank=0.902。GSTM1基因无缺失型与缺失型Cox比例风险系数比为1.07,95%CI为0.48~2.42,P=0.87。结论不同GSTP1和GSTM1基因型患者化疗后总生存差异无统计学意义,但应用TP方案化疗患者总生存率显著高于其他方案化疗患者。
[Abstract]:Objective to investigate the effects of single nucleotide polymorphisms of glutathione S-transferase (P1(glutathione S-transferase P1) and glutathione S-transferase (M1(glutathione S-transferase M1) gene on survival of patients with ovarian serous cystadenocarcinoma treated by chemotherapy. Methods 106 patients with gynecology of Linyi people's Hospital from August to January 2004.64 patients were treated with paclitaxel combined with taxol carboplatin TP1, and 42 patients with other chemotherapy regimen (topotecan, cyclophosphamide, isocyclophosphamide or gemcitabine). The single nucleotide polymorphisms of GSTP1 and GSTM1 were detected by pyrosequencing. GSTP1Ile105ValA GSTM1 deletion type / GSTM1 non-deletion type was detected. The association between genotype and overall survival was evaluated using the Kaplan-Meier survival curve and the Cox proportional risk regression model. Results the overall survival rate was 38.1% in TP group, significantly higher than that in other chemotherapy group (23.2g Plog-rank 0.006 GSTP 1). The overall survival rate of patients with alleleucine homozygous AP-A-1 genotype was 34.6GSTP1 (total survival rate 57.6%), the difference was 57.6% (P < 0.05). There was no statistical significance, 蠂 2 / 0.132 P log-rank-0.716 Cox proportional risk regression analysis showed that the risk coefficient ratio of GSTP 1 / A / G to allele A / A was 1.20 / 95 CI = 0.55 / 2.63 / P 0.65. The overall survival rate of patients without homozygous vector of GSTP1 valine allele was 29.5% and that of non-deletion type of GSTM1 was 74.9%. There was no significant difference between them. The proportional risk coefficient of non-deletion type and deletion type Cox was 1.0795 CI 0.48n2.42P0.87. Conclusion there was no significant difference in total survival of patients with different GSTP1 and GSTM1 genotypes after chemotherapy, but the overall survival rate of patients with TP regimen was significantly higher than that of patients with other chemotherapy regimens.
【作者单位】：山东省临沂卫生学校妇产科教研室;临沂市人民医院妇产科;
【分类号】：R737.31

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