基于大数据对宫颈癌缺氧生物标志物的分析

发布时间：2018-05-13 11:52

本文选题：宫颈肿瘤 + 缺氧　；参考：《中华疾病控制杂志》2017年05期

【摘要】：目的在GEO(gene expression omnibus)和TCGA(the cancer genome atlas)数据库中挖掘宫颈癌缺氧标志物并对其预后进行生存分析。方法利用GEO数据库中宫颈癌表达谱(GSE75034)缺氧组织和非缺氧组织中的数据进行差异性分析,再运用基因功能分析(gene ontology,GO)与通路富集分析(kyoto encyclopedia of genes and genomes,KEGG)筛选出相关的通路和相关功能的基因,结合TCGA数据库中宫颈癌的数据对其预后运用KaplanMeier与Log-rank检验方法进行生存分析。结果显示有337个基因表达存在差异,缺氧组织中上调的基因有184个,下调的有153个。GO分析显示这些基因与免疫反应、炎症反应、细胞增殖、血管形成等有关;KEGG分析显示主要富集在TNF信号通路、PI3K-Akt信号通路、HIF-1信号通路等。生存分析显示富集在HIF-1通路上的PAI-1、BCL-2、HK-2与宫颈癌病人预后相关,其中PAI-1、HK-2是预后的危险因素(均有P0.05),高表达组与患者生存期缩短相关,BCL-2是保护因素(X~2=6.508,P=0.011),高表达组与患者生存期延长相关。结论 PAI-1、BCL-2、HK-2可以作为宫颈癌缺氧生物标志物,与宫颈癌病人的预后相关,为后续的研究提供新思路。
[Abstract]:Objective to excavate the anoxic markers of cervical cancer in GEO (Gene Expression Omnibus) and TCGA (the Cancer Genome Atlas) database and to analyze the survival of the cervical cancer. Methods the data in the hypoxia and non anoxic tissues of the cervical cancer expression spectrum (GSE75034) in the GEO database were analyzed, and the gene functional analysis (gene) was used. Tology, GO) and pathway enrichment analysis (Kyoto Encyclopedia of genes and genomes, KEGG) screening genes related to pathways and related functions, combined with KaplanMeier and Log-rank test methods for survival analysis of cervical cancer in the TCGA database. The results showed that there were 337 differences in gene expression, in hypoxia tissue. There were 184 up-regulated genes, and 153.GO analyses showed that these genes were related to immune response, inflammatory response, cell proliferation, angiogenesis and so on; KEGG analysis showed that these genes were mainly enriched in TNF signaling pathway, PI3K-Akt signaling pathway, HIF-1 signaling pathway, etc. survival analysis showed that PAI-1, BCL-2, HK-2 and cervical cancer were enriched in the HIF-1 pathway. The prognosis is related, in which PAI-1 and HK-2 are the risk factors of prognosis (P0.05), the high expression group is related to the shortening of the patient's life, BCL-2 is the protective factor (X~2=6.508, P=0.011), and the high expression group is associated with the prolongation of the patient's life. Conclusion PAI-1, BCL-2, HK-2 can be used as a biomarker for the hypoxia of cervical cancer, and is related to the prognosis of cervical cancer patients. The follow-up research provides a new way of thinking.

【作者单位】：广西医科大学公共卫生学院流行病学教研室;
【基金】：国家自然科学基金(81241100)
【分类号】：R737.33

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