原因不明复发性流产患者外周血单核细胞上Tim-3和PD-1的表达及意义

发布时间：2018-05-13 18:08

本文选题：Tim- + PD-　；参考：《免疫学杂志》2017年04期

【摘要】：目的探讨原因不明复发性流产(unexplained recurrent spontaneous abortion,URSA)和先兆流产患者外周血单核细胞上PD-1和Tim-3的表达及其细胞因子的分泌情况,为先兆流产和URSA发病机制研究提供理论依据。方法随机选取29例健康早孕妇女作为正常早孕组(A组),37例仅有少量阴道出血的早孕妇女为先兆流产组(B组),22例URSA早孕患者为URSA组(C组);应用密度梯度离心法分离外周血单个核细胞,然后采用流式细胞分析技术检测3组孕妇外周血中CD14+单核细胞上Tim-3、PD-1的表达水平;ELISA法检测外周血中IL-4、IL-12、IFN-γ的含量。结果与正常早孕组相比,先兆流产组和URSA组外周血单核细胞上Tim-3、PD-1表达降低,外周血IL-4表达降低,URSA组低于先兆流产组(P0.05);IFN-γ、IL-12表达增高,URSA组高于先兆流产组(P0.05);相关性分析提示先兆流产组及URSA组外周血单核细胞上Tim-3与PD-1之间呈正相关;Tim-3、PD-1与IL-12呈负相关,IL-12与IL-4呈负相关,与IFN-γ呈正相关。结论外周血单核细胞上Tim-3、PD-1可能通过调节IL-12产生间接调节Th1/Th2平衡参与先兆流产和URSA的发生。
[Abstract]:Objective to investigate the expression of PD-1 and Tim-3 and cytokine secretion in peripheral blood monocytes of patients with unexplained recurrent spontaneous abortion (Ursa) and threatened abortion, and to provide theoretical basis for the study of pathogenesis of threatened abortion and URSA. Methods Twenty-nine healthy early pregnant women were randomly selected as normal early pregnancy group (group A), 37 early pregnant women with only a small amount of vaginal bleeding were selected as threatened abortion group (group B) and 22 patients with URSA early pregnancy as URSA group (group C), and were separated by density gradient centrifugation. Peripheral blood mononuclear cells, Then the expression level of Tim-3P PD-1 on CD14 monocytes in three groups of pregnant women was detected by flow cytometry and the level of IL-4 IL-12 IFN- 纬 in peripheral blood was detected by Elisa. Results compared with normal early pregnancy group, the expression of Tim-3 PD-1 on peripheral blood monocytes in threatened abortion group and URSA group was decreased. The decrease of IL-4 expression in peripheral blood in Ursa group was lower than that in threatened abortion group and the expression of IFN- 纬 -IL-12 in Ursa group was higher than that in threatened abortion group, and the correlation analysis indicated that there was a positive correlation between Tim-3 and PD-1 in peripheral blood monocyte of threatened abortion group and URSA group. There was a negative correlation between IL-12 and IL-4. There was a positive correlation with IFN- 纬. Conclusion Tim-3 PD-1 may be involved in the occurrence of threatened abortion and URSA by regulating the production of IL-12 and indirectly regulating the balance of Th1/Th2 in peripheral blood monocytes.
【作者单位】：大同大学免疫学研究所;大同大学医学院外科实验室;
【基金】：国家自然科学基金(81170621)
【分类号】：R714.21

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