MicroRNA-20b在卵巢癌细胞中的表达及对增殖和凋亡的影响

发布时间：2018-05-13 21:36

本文选题：miR-b + 卵巢癌　；参考：《中国现代医学杂志》2017年13期

【摘要】：目的探讨micro RNA-20b(miR-20b)在卵巢癌细胞系中的表达及对增殖和凋亡的影响。方法实时荧光定量聚合酶链反应(q RT-PCR)测定miR-20b在卵巢癌细胞系A431、SKOV3及正常卵巢上皮细胞系Hose中的相对表达量,将SKOV3细胞系分成未转染对照、阴性对照及miR-20b模拟物组,用Lipofectamine2000分别不转染、转染miR-20b scramble和miR-20b mimics,CCK8实验测定3组细胞增殖能力,流式细胞术测定3组细胞凋亡,Western blot测定张力蛋白同源在10号染色体有缺失的磷酸酶(PTEN)、裂解型聚腺苷二磷酸核糖聚合酶(PARP)的相对表达量。结果 q RT-PCR示A431、SKOV3细胞系miR-20b相对表达量较Hose细胞系低;miR-20b模拟物组在0、24、48、72及96 h的OD45 nm值低于未转染对照组和阴性对照组;miR-20b模拟物组凋亡率高于阴性对照组和未转染对照组;miR-20b模拟物组PTEN相对表达量低于阴性对照组,裂解型PARP蛋白相对表达量高于阴性对照组。结论 miR-20b抑制卵巢癌细胞增殖并促进凋亡,其机制可能与下调PTEN和上调PARP蛋白表达有关。
[Abstract]:Objective to investigate the expression of micro RNA-20bmmiR-20b in ovarian cancer cell line and its effect on proliferation and apoptosis. Methods the relative expression of miR-20b in ovarian cancer cell line A431SKOV3 and normal ovarian epithelial cell line Hose was determined by real-time fluorescence quantitative polymerase chain reaction (RT-PCR). The SKOV3 cell lines were divided into three groups: untransfected control, negative control and miR-20b mimics. The proliferative ability of the three groups was measured by miR-20b scramble and miR-20b mimicsl CCK8 transfection without Lipofectamine2000 transfection, respectively. Flow cytometry was used to determine the relative expression of PTEN, a phosphatase homologous to tensin homologue on chromosome 10, and a cleavage polyadenosine diphosphate polymerase (PARP). Results Q RT-PCR showed that the relative expression of miR-20b in A431 SKOV3 cell line was lower than that in Hose cell line. The OD45 nm value of the mimetic group was lower than that of the untransfected control group and the negative control group at 72 h and 96 h. The apoptotic rate of A431 SKOV3 cell line was higher than that of the negative control group and the untransfected control group. The relative expression of PTEN in the miR-20b analogue group was lower than that in the negative control group. The relative expression of cleavage type PARP protein was higher than that of negative control group. Conclusion miR-20b inhibits proliferation and promotes apoptosis of ovarian cancer cells, which may be related to down-regulation of PTEN and up-regulation of PARP protein expression.
【作者单位】：湖北省武汉市第一医院妇产科;
【基金】：武汉市卫计委课题(No:WX15C32)
【分类号】：R737.31

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