PTEN在子痫前期胎盘滋养细胞浸润不足中的作用及机制研究

发布时间：2018-05-14 12:48

本文选题：PTEN + 子痫前期　；参考：《现代妇产科进展》2017年10期

【摘要】：目的:检测子痫前期(PE)患者胎盘组织中PTEN表达,探讨PTEN对滋养细胞迁移功能的调控及其可能机制。方法:收集重度PE患者和正常孕妇的胎盘组织各20例,实时定量PCR和Western blot法检测胎盘组织中PTEN表达;将PTEN过表达质粒(pcDNA3.1-HA-PTEN)或特异性siRNA(siPTEN)瞬时转染至HTR-8/SVneo细胞,同时转染pcDNA3.1或siCTL作为对照。采用划痕实验评估细胞迁移能力,Western blot法检测p-Akt(Thr308)、p-Akt(Ser473)、Akt、MMP-2和MMP-9表达。结果:重度PE患者胎盘组织中PTEN mRNA和蛋白表达明显高于正常孕妇,差异均有统计学意义(P0.01)。与转染pcDNA3.1组比较,pcDNA3.1-HA-PTEN瞬时转染HTR-8/SVneo细胞48h后,细胞迁移率明显降低[(26.42±6.68)%vs(52.92±6.71)%,P0.01],p-Akt(Thr308)和p-Akt(Ser473)表达显著下调,Akt表达无明显改变,MMP-2和MMP-9蛋白表达下降;与转染siCTL组比较,siPTEN瞬时转染HTR-8/SVneo细胞48h后,细胞迁移率则明显升高[(50.39±6.84)%vs(30.04±5.40)%,P0.01],p-Akt(Thr308)和p-Akt(Ser473)的表达明显上调,Akt表达无明显改变,MMP-2和MMP-9蛋白表达升高。结论:重度PE患者胎盘组织中PTEN表达明显升高,PTEN可通过下调Akt活性降低MMP-2和MMP-9表达,抑制滋养细胞的迁移。提示PTEN在PE的发病中发挥了一定的作用。
[Abstract]:Aim: to investigate the expression of PTEN in placenta of patients with preeclampsia and to explore the regulation of trophoblast migration by PTEN and its possible mechanism. Methods: the placental tissues of 20 patients with severe PE and 20 normal pregnant women were collected to detect the expression of PTEN in placenta tissue by real-time quantitative PCR and Western blot, and the PTEN overexpression plasmid pcDNA3.1-HA-PTEN) or specific siRNAsiPTEN) was transfected into HTR-8/SVneo cells. At the same time, pcDNA3.1 or siCTL were transfected as control. The cell migration ability was evaluated by scratch assay. The expression of p-Akttttttsil, Thr308, p-AktSer 473 and AktTU MMP-2 and MMP-9 were detected by Western blot assay. Results: the expression of PTEN mRNA and protein in placenta of severe PE patients was significantly higher than that of normal pregnant women (P 0.01). Compared with the pcDNA3.1 transfection group, the cell migration rate of HTR-8/SVneo cells transfected with pcDNA3.1-HA-PTEN for 48h was significantly decreased [26.42 卤6.68)%vs(52.92 卤6.71p0.01] and p-Akttsi-Thr308), and the expression of MMP-2 and MMP-9 protein was not significantly decreased after transfection of siCTL, and the expression of MMP-2 and MMP-9 protein was decreased after transient transfection of HTR-8/SVneo cells 48 h after transfection of siCTL, and the expression of MMP 2 and MMP-9 protein decreased significantly after transfection of ppcDNA3.1-HA-PTEN, and the expression of MMP 2 and MMP-9 protein decreased significantly after transfection of ppcDNA3.1-HA-PTEN. The cell migration rate was significantly increased [50.39 卤5.40 6.84)%vs(30.04 卤5.40 + P0.01] and p-AktTU Thr308 and p-AktTU Ser473) were significantly up-regulated. No significant changes in the expression of MMP-2 and MMP-9 protein were observed. Conclusion: the expression of PTEN in placenta of severe PE patients was significantly increased. PTEN could decrease the expression of MMP-2 and MMP-9 and inhibit the migration of trophoblast by down-regulating the activity of Akt. The results suggest that PTEN may play a role in the pathogenesis of PE.
【作者单位】：南京医科大学附属常州妇幼保健院妇产科;南京医科大学附属常州第二人民医院乳腺外科;南京大学医学院附属鼓楼医院妇产科;
【基金】：南京医科大学科技发展基金重点项目(No:2016NJMUZD095)
【分类号】：R714.244

【相似文献】