孕期炎症暴露致子代大鼠肾脏组织中甲基化转移酶表达异常的研究

发布时间：2018-05-15 00:33

本文选题：炎症 + 脂多糖类　；参考：《重庆医学》2015年13期

【摘要】：目的探讨肾脏DNA甲基转移酶(DNMTs)在孕期脂多糖(LPS)刺激仔鼠高血压发生的作用。方法将孕鼠分为4组,分别为对照组(孕期的8~14d注射生理盐水)、LPS组(孕期第8、10、12天注射LPS,在9、11、13、14天注射生理盐水)、核转录因子κB(NF-κB)抑制剂PDTC组(孕期的8~14d每天注射PDTC)、LPS+PDTC组(LPS在孕期第8、10、12天注射,PDTC注射时间及剂量同PDTC组)。用尾动脉无创血压测定仪测仔鼠血压,电子天平称体质量,RT-PCR检测仔鼠肾皮质白细胞介素-6(IL-6)、Fli-1及DNMTs的表达水平。结果LPS组仔鼠血压明显高于对照组(P0.05),体质量较对照组高,肾皮质IL-6、Fli-1、DNMT1、DNMT3b表达较对照组升高(P0.05)。而LPS+PDTC组仔鼠体质量、血压及IL-6、Fli-1、DNMT1、DNMT3b表达均较LPS组降低。结论孕期LPS刺激会引起仔鼠肾皮质DNA甲基酶变化,提示一些关键基因的甲基化可能是孕期LPS刺激引起子代高血压的原因之一。
[Abstract]:Objective to investigate the effects of renal DNA methyltransferase (DNA) on the development of hypertension in neonatal rats induced by lipopolysaccharide (LPS). Methods pregnant rats were divided into 4 groups. The rats in the control group (8 days after pregnancy) were injected with normal saline and lipopolysaccharide (LPSs) on the 12th day of pregnancy (810 days during pregnancy), the control group (PDTC group) with nuclear transcription factor 魏 BNF- 魏 B inhibitor (PDTC group) was injected with LPS on the 8th day of gestation, and the control group was injected with LPS on the 12th day of pregnancy at the 810th day of pregnancy, and the control group was injected with the inhibitor of nuclear transcription factor 魏 BNF- 魏 B on the 12th day of pregnancy. The injection time and dose of PDTC were the same as those of PDTC group. The blood pressure of young mice was measured by non-invasive blood pressure analyzer of caudal artery, and the expression of Fli-1 and DNMTs in renal cortex of young rats was detected by RT-PCR. Results the blood pressure in LPS group was significantly higher than that in control group (P 0.05), the body weight was higher than that in control group, and the expression of IL-6Fli-1DNMT1DNMT3b in renal cortex was higher than that in control group. The body weight, blood pressure and the expression of IL-6 Fli-1 DNMT1DNMT3b in LPS PDTC group were lower than those in LPS group. Conclusion LPS stimulation during pregnancy may cause changes of DNA methylase in renal cortex of neonatal rats, suggesting that methylation of some key genes may be one of the causes of hypertension in offspring induced by LPS stimulation during pregnancy.
【作者单位】：第三军医大学大坪医院野战外科研究所妇产科;第三军医大学药学院药剂学教研室;
【基金】：国家自然科学基金资助项目(81170580)
【分类号】：R714.2

【共引文献】