HLA-DQB1基因多态性与广西壮族女性HPV16感染和宫颈癌易感性的关联研究

发布时间：2018-05-16 07:30

本文选题：HLA-DQB + 宫颈癌　；参考：《中国免疫学杂志》2017年04期

【摘要】：目的:探讨HLA-DQB1等位基因多态性对广西壮族女性HPV16感染和宫颈癌发生的影响,为寻找广西壮族女性宫颈癌的遗传易感基因或保护基因提供线索。方法:选取广西地区25~45岁宫颈癌确诊的壮族患者、无癌健康壮族女性各171例作为研究对象(按年龄±3岁配对),采集研究对象样本并提取HPV核酸和人基因组DNA,分别应用PCR-SSP和分子导流杂交技术进行HLA-DQB1基因型检测和HPV基因分型检测,最后进行统计学分析。结果:(1)171例宫颈癌患者中HPV总感染率为91.22%,其中高危型病毒占90.76%,HPV16型为主要致病亚型(43.58%);(2)广西壮族女性宫颈癌患者组的HLA-DQB1*04等位基因携带率高于无癌对照组,差异具有统计学意义(P0.05);等位基因HLA-DQB1*06/09在宫颈癌患者组中的携带率明显低于无癌组,差异具有统计学意义(P0.05);而两组间的HLA-DQB1*02/05/07/08等位基因携带率无显著性差异(P0.05);(3)HLA-DQB1*04基因在HPV16阳性宫颈癌患者中出现的频率明显高于HPV16阴性患者,差异具有统计学意义(P0.05)。结论:HLA-DQB1*04可能是广西壮族女性宫颈癌发生的易感基因,HLA-DQB1*06/09可能是广西壮族女性宫颈癌的保护基因。而HLA-DQB1*02/05/07/08等位基因可能与广西壮族女性宫颈癌遗传易感性无关。携带HLA-DQB1*04等位基因的广西壮族妇女可能更容易感染HPV16型病毒,从而增加了其患宫颈癌的危险性。
[Abstract]:Objective: to investigate the effect of HLA-DQB1 allele polymorphism on HPV16 infection and cervical cancer in Zhuang women in Guangxi. Methods: selected 25 ~ 45 years old patients with cervical cancer in Guangxi, A total of 171 healthy Zhuang women (age 卤3 years old) were selected as the study subjects. HPV nucleic acid and human genomic DNA were collected, and HLA-DQB1 genotypes and HPV genotypes were detected by PCR-SSP and molecular diversion hybridization techniques, respectively. Finally, statistical analysis was carried out. Results the total infection rate of HPV in 171 patients with cervical cancer was 91.22. The rate of HLA-DQB1*04 alleles in Guangxi Zhuang women with cervical cancer was higher than that in non-cancer control group (90.76% HPV16 was the main pathogenic subtype of HPV16). The frequency of allelic HLA-DQB1*06/09 was significantly lower in patients with cervical cancer than that in patients without cervical cancer, the difference was statistically significant (P 0.05), and the frequency of allelic HLA-DQB1*06/09 was significantly lower in patients with cervical cancer. There was no significant difference in the frequency of HLA-DQB1*02/05/07/08 alleles between the two groups. The frequency of HLA-DQB1n04 gene in patients with HPV16 positive cervical cancer was significantly higher than that in patients with HPV16 negative, and the difference was statistically significant (P 0.05). Conclusion: HLA-DQB1O4 may be the susceptible gene of cervical cancer in Zhuang women in Guangxi. HLA-DQB1 / 06 / 09 may be a protective gene for cervical cancer in Zhuang women in Guangxi. The HLA-DQB1*02/05/07/08 allele may not be associated with the genetic susceptibility of cervical cancer in Zhuang women in Guangxi. Guangxi Zhuang women with HLA-DQB1*04 allele were more likely to be infected with HPV16, which increased their risk of cervical cancer.
【作者单位】：广西卫生职业技术学院;
【基金】：广西壮族自治区教育厅2016年度广西高校中青年教师基础能力提升项目(No.KY2016YB740) 广西壮族自治区教育厅项目(No.GXGZJG2015B240)
【分类号】：R737.33

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