瞬时感受器阳离子通道6与子痫前期发病的相关性研究

发布时间：2018-05-16 09:34

本文选题：先兆子痫 + 瞬时受体电位通道　；参考：《青岛大学》2014年硕士论文

【摘要】：目的探讨瞬时感受器阳离子通道6(transient receptor potential channel6, TRPC6)与子痫前期发病的关系。方法选取2012年8月-2013年1月在青岛大学附属医院产科住院的轻度子痫前期孕妇20例(轻度子痫前期组),重度子痫前期孕妇(重度子痫前期组)23例以及因社会因素和头盆不称因素剖宫产患者(对照组)25例。采用酶联免疫吸附试验(ELISA)检测三组孕妇血清中TRPC6的水平,并分析各组孕妇血清TRPC6与血压、生化指标和24h尿蛋白总量的相关性。采用荧光定量PCR检测上述各组受试者各组织(胎盘及子宫平滑肌)中TRPC6的mRNA含量。采用WESTEN-BLOT方法检测胎盘及子宫平滑肌组织中TRPC6的蛋白表达。并分析血清TRPC6与各组织TRPC6mRNA及蛋白的相关性。结果(1)血清TRPC6水平：轻度子痫前期组及重度子痫前期组均高于对照组,差异有显著性(F=5.98,q=3.43,q=4.68,P0.05)；轻度子痫前期组和重度子痫前期组水平差异无显著性(q=1.05,P0.05)。在轻度子痫前期组与重度子痫前期组中,血清TRPC6水平与血压(收缩压及舒张压均)呈正相关(r=0.712、0.774,P0.05)；与24h尿蛋白总量均呈正相关(r=0.841、0.897,P0.05)；与甘油三酯(TG)、总胆固醇(TC)、高密度脂蛋白(HDL)、低密度脂蛋白(LDL)均无相关性(P0.05)。(2)胎盘组织中TRPC6mRNA表达水平在轻度子痫前期组及重度子痫前期组孕妇中分别高于正常组的2.1倍和2.7倍,分别比较,差异均有统计学意义(F=9.99,q=4.88,q=5.90,P0.05)；子宫平滑肌中TRPC6mRNA表达水平在轻度组及重度组孕妇中分别高于正常组的6倍和10倍,分别比较,差异均有统计学意义(F=41.38,q=9.49,q=12.38,P0.05)；胎盘和子宫平滑肌的TRPC6mRNA表达水平,在轻度组和重度组中的差异无显著性(q=0.79、2.34,P0.05)。(3)胎盘组织中TRPC6蛋白在轻度子痫前期组及重度子痫前期组孕妇的表达水平分别为0.86±0.33,1.09±0.302,明显高于对照组的0.54±0.254,差异有显著性(F=10.08,q轻,对=5.08,q重,对=9.17,q轻,重=3.59,P0.05)；子宫平滑肌中TRPC6蛋白的表达水平分别为1.00±0.175,1.18±0.134,明显高于对照组的0.65±0.245,差异有显著性(F=20.10,q轻,对=8.75,q重,对=13.59,q轻,重=4.39,P0.05)(4)血清TRPC6水平与胎盘的TRPC6mRNA及蛋白均呈正相关(r=0.31,r=1.37,P0.05),血清TRPC6水平与子宫平滑肌的TRPC6mRNA无相关性(r=0.11,P0.05),与子宫平滑肌的TRPC6蛋白呈正相关(r=1.36,P0.05)。结论子痫前期患者胎盘组织及子宫平滑肌中TRPC6mRNA表达水平上调及TRPC6蛋白表达增加,可能引起血清中TRPC6水平升高。血管内皮细胞受损,导致的血管平滑肌细胞上TRPC6通道激活可能与子痫前期发生发展有关。
[Abstract]:Objective to investigate the relationship between transient receptor cationic channel 6(transient receptor potential channel 6 (TRPC6) and preeclampsia. Methods from August 2012 to January 2013, 20 pregnant women with mild preeclampsia (mild preeclampsia), severe preeclampsia (23 severe preeclampsia) and social factors were selected. And cephalopelvic disproportion factor cesarean section (control group, 25 cases). The levels of TRPC6 in serum of three groups of pregnant women were detected by Elisa, and the correlation between serum TRPC6 and blood pressure, biochemical index and total amount of 24 hours urine protein was analyzed. The content of TRPC6 mRNA in placenta and uterine smooth muscle was measured by fluorescence quantitative PCR. The expression of TRPC6 protein in placenta and uterine smooth muscle tissue was detected by WESTEN-BLOT method. The correlation between serum TRPC6 and TRPC6mRNA and protein was analyzed. Results 1) the level of serum TRPC6 in mild preeclampsia group and severe preeclampsia group was higher than that in control group, the difference was significant (P 0.05), and there was no significant difference between mild preeclampsia group and severe preeclampsia group. In the mild preeclampsia group and the severe preeclampsia group, Serum TRPC6 levels were positively correlated with blood pressure (systolic blood pressure and diastolic blood pressure), 0.712 ~ 0.774U / P 0.05, r ~ (0.841) / 0.897p _ (0.05), respectively, and had no correlation with triglyceride (TG), total cholesterol (TC), high density lipoprotein (HDL), low density lipoprotein (LDLs), and placenta group (P _ (0.05) n ~ (-1). The expression of TRPC6mRNA in pregnant women with mild preeclampsia and severe preeclampsia was 2. 1 and 2. 7 times higher than that of normal women, respectively. The difference was statistically significant (P 0.05), and the expression of TRPC6mRNA in the uterine smooth muscle was 6 times and 10 times higher in the mild and severe pregnant women than in the normal group, respectively. The difference was statistically significant (P < 0.05). The expression of TRPC6mRNA in placenta and uterine smooth muscle was significantly higher than that in normal placenta and uterus. There was no significant difference between mild group and severe group. The expression of TRPC6 protein in placenta tissue was 0.86 卤0.33 1.09 卤0.302 in mild preeclampsia group and severe preeclampsia group respectively, which was significantly higher than that in control group (0.54 卤0.254). There was significant difference in the expression of TRPC6 protein between mild preeclampsia group and severe preeclampsia group (P < 0.05), and the difference was significant (P < 0.05), and the expression of TRPC6 protein in pregnant women with mild preeclampsia and severe preeclampsia was 0.86 卤0.31.09 卤0.302, respectively, which was significantly higher than that of control group (0.54 卤0.254). The expression levels of TRPC6 protein in uterine smooth muscle were 1.00 卤0.175 ~ 1.18 卤0.134, respectively, which were significantly higher than those in the control group (0.65 卤0.245). The difference was significant (P < 0.05). The expression of TRPC6 protein in uterine smooth muscle was significantly higher than that in the control group (0.65 卤0.245). There was a positive correlation between serum TRPC6 level and placental TRPC6mRNA and protein. There was no correlation between serum TRPC6 level and TRPC6mRNA of uterine smooth muscle, and there was a positive correlation between serum TRPC6 level and TRPC6 protein of uterine smooth muscle. Conclusion the expression of TRPC6mRNA and the expression of TRPC6 protein in placenta and uterine smooth muscle of preeclampsia patients are up-regulated, which may lead to the increase of TRPC6 level in serum. The activation of TRPC6 channels in vascular smooth muscle cells may be related to the development of preeclampsia.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R714.244

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