液质谱联用法同时检测体外生殖培养液中的3种能量成分

发布时间：2018-05-18 07:55

  本文选题：葡萄糖 + 乳酸钠　； 参考：《中国组织工程研究》2017年02期

【摘要】：背景:目前,对于体外生殖培养液的质量控制文件或技术标准中,对成分含量的指标及检测方法未做明确规定,为保证该类产品的安全有效性,应及早建立质量标准。目的:建立采用液相质谱联用法检测体外生殖培养液中葡萄糖、乳酸钠、丙酮酸钠3种指标成分的方法,分析不同用途培养液中3种成分的含量。方法:采用超高效液相色谱三重四级杆质谱联用法,使用SUPELCO Discovery HS F5-3色谱柱(15 cm×2.1 mm,3μm),流动相为0.1%甲酸水溶液-0.1%甲酸乙腈溶液,梯度洗脱,流速0.35 m L·min-1,柱温40℃;质谱采用电喷雾离子源,负离子模式扫面,扫描方式为多反应监测。结果与讨论:葡萄糖、丙酮酸钠、乳酸钠分别在0.1-10 mg/L(r=0.999 8)、0.05-5 mg/L(r=0.999 4)、0.1-10 mg/L(r=0.999 4)范围线性关系练好;平均加样回收率在96.4%-98.1%之间,相对标准偏差均小于2.8%。说明采用液相质谱联用法检测体外生殖培养液灵敏、快速、准确、专属性强,可为体外生殖培养液的质量标准研究提供依据。
[Abstract]:Background: at present, in the quality control document or technical standard of in vitro reproductive culture medium, there is no clear stipulation about the index of the content of ingredients and the detection method. In order to ensure the safety and effectiveness of this kind of product, the quality standard should be established as soon as possible. Objective: to establish a method for the determination of glucose, sodium lactate and sodium pyruvate in vitro reproductive culture medium by liquid phase mass spectrometry, and to analyze the contents of three components in different culture media. Methods: a SUPELCO Discovery HS F5-3 column of 15cm 脳 2.1mm ~ (-3) 渭 m ~ (-1) was used. The mobile phase consisted of 0.1% formic acid aqueous solution -0.1% acetonitrile formate solution, gradient elution, flow rate 0.35 mL 路min ~ (-1), column temperature 40 鈩，

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