Caspase-9在子痫前期患者胎盘中的表达及意义

发布时间：2018-05-19 14:39

本文选题：子痫前期 + caspase-9　；参考：《山西医科大学》2014年硕士论文

【摘要】：目的：子痫前期是一种严重威胁母婴健康的妊娠期特发性疾病，其发病机制至今尚未完全阐明。有研究发现滋养细胞的过度凋亡可能导致子痫前期的发生，，而caspase家族是重要的凋亡蛋白，其中caspase-9作为凋亡的起始环节起着关键性的作用，因此本文主要探讨caspase-9在子痫前期重度患者胎盘组织中的表达和意义。方法：选取2013年4月～2013年12月于山西医科大学第二临床医院妇产科住院分娩的孕妇，其中25例子痫前期重度患者作为研究组,另外选取25例正常晚期妊娠妇女作为正常对照组,收集患者全部临床信息和分娩时胎盘组织。用免疫组织化学二步法检测caspase-9在胎盘组织中的定位及表达,并用BI-2000图像分析系统进行图处理;western-blot方法检测caspase-9在胎盘组织中的定量表达。统计分析采用SPSS16.0软件，所有数据均用均数±标准差表示。两组间的临床数据和不同指标表达之间的差异均采用两独立样本t检验进行评价，以α＝0.05为研究水准，P＜0.05为差异有统计学意义。结果：1.两组研究资料的比较：研究组与正常对照组在年龄及孕龄上无显著性差异（P＞0.05）；在收缩压、舒张压、新生儿体重、新生儿评分上有显著性差异（P＜0.05）。 2.免疫组化检测结果：研究组和正常对照组在胎盘滋养细胞的胞质中均可见caspase-9的阳性表达,且在子痫前期组胎盘滋养细胞中caspase-9的表达(OD：0.322±0.04)显著高于正常对照组(OD：0.258±0.05)，差异有显著性(t＝-5.227，P＜0.01)。 3. Western-blot检测结果:子痫前期组胎盘滋养细胞中caspase-9的表达(灰度值：2.58±0.47)高于正常对照组(灰度值：1.66±0.19)，差异有显著性（t＝-3.693,P＜0.05）。结论：caspase-9表达于胎盘滋养细胞的胞质中，且在子痫前期重度患者胎盘滋养细胞的胞质中的表达升高，提示在妊娠妇女胎盘组织中caspase-9作为一种凋亡蛋白，它的表达水平可能与子痫前期的发病有关。
[Abstract]:Objective: preeclampsia is a serious threat to maternal and child health, gestational idiopathic disease, its pathogenesis has not been fully elucidated. Some studies have found that excessive apoptosis of trophoblast may lead to preeclampsia, and caspase family is an important apoptotic protein, in which caspase-9 plays a key role in the initiation of apoptosis. Therefore, we mainly studied the expression and significance of caspase-9 in placenta of severe preeclampsia. Methods: from April 2013 to December 2013, 25 pregnant women with severe preeclampsia and 25 normal women with late pregnancy were selected as the control group, who were given birth in gynecology and obstetrics department of the second Clinical Hospital of Shanxi Medical University from April 2013 to December 2013. Collect all clinical information and placental tissue during delivery. The localization and expression of caspase-9 in placenta were detected by immunohistochemical two-step method, and the quantitative expression of caspase-9 in placenta was detected by BI-2000 image analysis system. SPSS16.0 software is used for statistical analysis, and all data are represented by mean 卤standard deviation. The differences of clinical data and expression of different indexes between the two groups were evaluated by two independent samples t test, and the difference was statistically significant with 伪 -0.05 as the research level (P < 0.05). The result is 1: 1. There was no significant difference in age and gestational age between the study group and the normal control group (P > 0.05), but there was significant difference in systolic blood pressure, diastolic blood pressure, neonatal weight and neonatal score (P < 0.05). 2. The results of immunohistochemistry: the positive expression of caspase-9 was found in the cytoplasm of placental trophoblast in both study group and normal control group, and the expression of caspase-9 in placental trophoblastic cells in preeclampsia group was significantly higher than that in normal control group (OD0.322 卤0.04), and the difference was significant (P < 0.01). 3. Results of Western-blot: the expression of caspase-9 in placental trophoblast of preeclampsia group (grayscale: 2.58 卤0.47) was higher than that of normal control group (grayscale: 1.66 卤0.19, P < 0.05). Conclusion the results suggest that caspase-9 is a kind of apoptotic protein in placenta of pregnant women, and it is expressed in the cytoplasm of placental trophoblastic cells and in the cytoplasm of placental trophoblast of severe preeclampsia. Its expression may be associated with preeclampsia.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R714.244

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