XIAP、VEGF在子痫前期中的表达及相关性研究

发布时间：2018-05-19 16:41

本文选题：子痫前期 + XIAP　；参考：《广西医科大学》2014年硕士论文

【摘要】：目的：探讨分析XIAP、VEGF在子痫前期组孕妇与对照组孕妇血清、胎盘组织中的表达情况，研究其对子痫前期的影响及二者的相关性，为子痫前期临床治疗及科研工作提供依据。方法：选取30例2012年11月至2013年7月在广西医科大学第一附属医院住院孕产妇中，子痫前期的患者30例，其中20例轻度子痫前期患者，10例重度子痫前期患者。随机抽选同期妊娠晚期的正常孕妇共20例做为对照组。采集病例的临床特征资料进行比较；采用亲和免疫细胞化学技术链霉素－生物素－过氧化物酶复合物技术（SABC）法测定胎盘组织中XIAP、VEGF蛋白的表达。通过ELISA技术对XIAP、VEGF在正常妊娠孕妇和子痫前期孕妇外周血中的表达含量进行测定。结果：（1）子痫前期患者血清中XIAP、VEGF表达水平分别为（203.92士19.97）mg/L、（116.32士11.86）mg/L，对照组孕妇血清中XIAP、VEGF表达水平分别为（300.88士25.50）mg/L、（164.47士15.47）mg/L。XIAP、VEGF在子痫前期组和正常对照组血清中的表达量差异具有统计学意义(P均＜0.05)；XIAP、VEGF在轻度子痫前期组血清中的表达量分别为（215.56士9.60）mg/L、（122.81士8.24）mg/L，XIAP、VEGF在重度子痫前期组血清中的表达量分别为（180.62士13.66）mg/L、(103.32士5.35）mg/L，轻、重度子痫前期组血清中XIAP、VEGF相对表达量差异均有统计学意义，XIAP、VEGF在血清中的表达量呈正相关（r=0.929）。（2）XIAP在对照组、轻度和重度子痫前期组胎盘组织中的阳性表达率分别为100%、84%和62%，其中轻度子痫前期组与对照组相比，差异有统计学意义(χ2=17.391，P=0.000)，重度子痫前期组与轻度子痫前期组相比，差异有统计学意义(χ2=9.019，P=0.003)；VEGF在对照组、轻度和重度子痫前期组胎盘组织中的阳性表达率分别为100%、91%和72%，其中轻度患者组与对照组相比，差异有统计学意义(χ2=10.497，，P=0.001)，重度患者组与轻度患者组相比，差异有统计学意义(χ2=9.269，P=0.002)。结论： XIAP、VEGF可能参与了子痫前期的发生发展。
[Abstract]:Objective: to investigate the expression of XIAPP VEGF in serum and placenta of pregnant women with preeclampsia and control group, and to study its effect on preeclampsia and the correlation between them, and to provide the basis for clinical treatment and scientific research of preeclampsia. Methods: from November 2012 to July 2013, 30 pregnant women with pre-eclampsia were enrolled in the first affiliated Hospital of Guangxi Medical University, including 20 patients with mild pre-eclampsia and 10 patients with severe pre-eclampsia. Twenty normal pregnant women in the third trimester of pregnancy were randomly selected as control group. The expression of VEGF protein in placenta was determined by streptomycin-biotin-peroxidase complex technique. The expression of ELISA in peripheral blood of normal pregnant women and preeclampsia pregnant women was determined by ELISA technique. Results the expression level of XIAPN VEGF in serum of preeclampsia patients was 203.92 卤19.97 mg / L, respectively. The expression level of XIAPN VEGF in serum of pregnant women in control group was 300.88 + 25.50 mg / L, respectively. The expression of XIAPN VEGF in serum of preeclampsia group was different from that of normal control group (P < 0.05). There was a significant difference between preeclampsia group and normal control group in the expression of XIAPP VEGF in serum of preeclampsia group and normal control group. The expression of VEGF in the serum of mild preeclampsia group was 215.56 卤9.60 mg / L 122.81 卤8.24 mg / L respectively. The expression of VEGF in the serum of severe preeclampsia group was 180.62 卤13.66 mg / L 103.32 卤5.35 mg / L, respectively. There were significant differences in the relative expression of XIAPP VEGF in serum of severe preeclampsia group. There was a positive correlation between the expression of XIAPP VEGF in serum and the level of XIAP in the control group. The positive expression rates of placenta in mild and severe preeclampsia groups were 100% and 62%, respectively. There was significant difference between the mild preeclampsia group and the control group (蠂 2 / 17.391P 0.000), and between the severe preeclampsia group and the mild preeclampsia group, there was a significant difference between the severe preeclampsia group and the mild preeclampsia group. The positive expression rates of VEGF in placenta of mild and severe preeclampsia groups were 100% and 72%, respectively. The positive rates of VEGF in mild and severe preeclampsia groups were 100% and 72%, respectively. The positive expression rates of VEGF in mild and severe preeclampsia groups were significantly higher than those in control group. The difference was statistically significant (蠂 ~ 2 + 10.497) P ~ (0.001), and there was a significant difference between the severe group and the mild group (蠂 ~ (2 +) 9.269% P ~ (0.002). Conclusion: VEGF may be involved in the development of preeclampsia.
【学位授予单位】：广西医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R714.244

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