基于母胎胆汁酸代谢轮廓的妊娠肝内胆汁淤积症分度研究

发布时间：2018-05-22 08:20

本文选题：妊娠肝内胆汁淤积症 + 胆汁酸代谢轮廓　；参考：《重庆医科大学学报》2016年03期

【摘要】：目的:探讨基于母胎血清胆汁酸代谢轮廓的妊娠肝内胆汁淤积症(intrahepatic cholestasis of pregnancy,ICP)分度。方法:采用高效液相色谱-紫外检测法对61名正常孕妇,轻、重度ICP患者母、脐血的9种结合型胆汁酸成分进行测定,并将受试者分为建模集(n=40)与验证集(n=21),结合偏最小二乘判别分析对胆汁酸代谢轮廓进行分析、建模与验证。结果:母血胆汁酸代谢模型可区分正常孕妇组,轻度ICP组,与重度ICP组;脐血胆汁酸代谢模型可区分重度ICP组与非重度组。母、脐血分度模型的验证效率分别达90.5%与100.0%。牛磺熊去氧胆酸(P=0.004),牛磺胆酸(P=0.005)以及甘氨胆酸(P=0.032)在ICP孕妇中明显增高;甘氨石胆酸(P0.001)和牛磺脱氧胆酸(P=0.001)在ICP胎儿中明显增高。结论:ICP母、脐血各有其特征的胆汁酸代谢轮廓,均可用于对ICP分度。
[Abstract]:Objective: to study the intrahepatic cholestasis of precancerous cholestasis in intrahepatic cholestasis of pregnancy based on the outline of maternal serum bile acid metabolism. Methods: nine kinds of binding bile acids in umbilical cord blood of 61 normal pregnant women with mild and severe ICP were determined by high performance liquid chromatography-ultraviolet detection. The subjects were divided into two groups: modeling and verification. The profile of bile acid metabolism was analyzed with partial least squares discriminant analysis. Results: maternal bile acid metabolism model could distinguish normal pregnant women from mild ICP group and severe ICP group, and umbilical blood bile acid metabolism model could distinguish severe ICP group from non-severe group. The validation efficiency of the model was 90.5% and 100.0%, respectively. Taurocholic acid (P0. 004), taurocholic acid (P0. 005) and glycosaminocholic acid (P0. 032) were significantly increased in pregnant women with ICP, while glycylcholic acid (P0. 001) and taurodeoxycholate (P0. 001) were significantly increased in ICP fetuses. Conclusion the metabolic profiles of bile acids in female and umbilical cord blood have their own characteristics, which can be used to classify ICP.
【作者单位】：重庆医科大学检验医学院临床检验诊断学教育部重点实验室;重庆医科大学公共卫生与管理学院卫生统计学教研室;重庆医科大学附属第一医院妇产科;
【基金】：国家自然科学基金资助项目(编号:81401228,81471473) 教育部高等学校博士学科点专项科研基金(博导类)资助项目(编号:20115503110013)
【分类号】：R714.25

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