PAX1基因甲基化定量检测在宫颈癌癌前病变诊断中的应用价值

发布时间：2018-05-22 12:33

本文选题：宫颈癌癌前病变 + 焦磷酸测序　；参考：《中国医学前沿杂志(电子版)》2016年12期

【摘要】：目的探讨PAX1基因甲基化定量检测在宫颈癌癌前病变诊断中的应用价值。方法选取2013年1月至2015年10月就诊于上海交通大学附属第六人民医院的121例高危亚型人乳头状瘤病毒(human papilloma virus,HPV)感染患者为研究对象。根据病理学结果将其分为正常宫颈组(28例)、低级别病变组[宫颈上皮内瘤变(cervical intraepi-thelial neoplasia,CIN)Ⅰ,32例]、高级别病变组(CINⅡ~Ⅲ,34例)及癌变组[浸润性宫颈癌(invasive cervical cancers,ICC),27例]。收集各组患者的宫颈组织标本及宫颈刮片,采用反转录聚合酶链反应和焦磷酸测序技术定量分析PAX1基因9个位点的甲基化水平,并通过接受者操作特征曲线(ROC曲线)计算PAX1甲基化与各级别宫颈病变的关联性。结果癌变组、高级别病变组、低级别病变组、正常宫颈组PAX1基因9个位点的甲基化平均水平分别为4.66%、3.55%、2.10%、1.90%,组间比较差异均具有显著性(P0.05)。各组患者宫颈刮片和宫颈组织PAX1甲基化水平比较差异均具有显著性(P0.01),其中,癌变组PAX1甲基化发生率及水平均显著高于其他三组(P0.05)。通过PAX1基因甲基化水平鉴别诊断宫颈癌和CIN,结果显示其ROC曲线下面积为0.88(P0.001),具有较高的诊断能力。结论定量检测宫颈组织中PAX1基因的甲基化水平能够有效诊断高级别宫颈癌前病变,且其表达与疾病分期有关。
[Abstract]:Objective to evaluate the value of quantitative detection of PAX1 gene methylation in the diagnosis of cervical precancerous lesions. Methods from January 2013 to October 2015, 121 patients with high risk human papillomavirus papilloma virus (HPV) infection in the sixth people's Hospital affiliated to Shanghai Jiaotong University were selected as the study subjects. According to the pathological results, it was divided into normal cervical carcinoma group (n = 28), low grade lesion group (n = 32), cervical intraepi-thelial neoplasia neoplasm group (n = 32), high grade lesion group (n = 34) and invasive cervical cancer group (n = 27). Cervical tissue samples and cervical scrapers were collected from patients in each group. The methylation levels of 9 PAX1 loci were analyzed by reverse transcriptase polymerase chain reaction (RT-PCR) and pyrosequencing. The correlation between PAX1 methylation and cervical lesions was calculated by the receiver operating characteristic curve (ROC curve). Results the average methylation level of PAX1 gene in cancer group, high grade lesion group, low grade lesion group and normal cervix group was 4.66 / 3.553.55 / 2.10 and 1.90 respectively. The difference between the two groups was significant (P 0.05). The levels of PAX1 methylation in cervical scraper and cervical tissue were significantly higher in each group than in other three groups (P 0.01). The incidence and level of PAX1 methylation in cancer group were significantly higher than those in other three groups (P 0.05). The PAX1 gene methylation level was used to differentiate cervical cancer from cin. The results showed that the area under the ROC curve was 0.88 P0.001 and had a high diagnostic ability. Conclusion quantitative detection of methylation level of PAX1 gene in cervical tissue can effectively diagnose high grade cervical precancerous lesions, and its expression is related to disease stage.
【作者单位】：上海交通大学附属第六人民医院妇产科;复旦大学附属闵行医院妇产科;
【基金】：上海市浦东新区卫生系统优秀青年医学人才培养计划(PWRq2013-16)
【分类号】：R737.33

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