AGEs及其受体与妊娠期糖尿病氧化应激相关性

发布时间：2018-05-22 13:51

本文选题：糖尿病 + 妊娠　；参考：《齐鲁医学杂志》2016年05期

【摘要】：目的探讨妊娠期糖尿病(GDM)孕妇血清及胎盘组织中晚期糖基化终产物(AGEs)及AGE受体(RAGE)的表达水平与氧化应激的相关性。方法选取2013年3月—2014年5月在我院产科行常规产前检查并分娩的GDM孕妇80例(GDM组)作为研究对象,另选择同期年龄、孕周、孕前BMI相似的正常孕妇80例作为对照组。采用酶联免疫吸附试验(ELISA)方法,分别检测两组孕妇孕24~26及37~39周血清及胎盘组织裂解液中AGE、可溶性RAGE(sRAGE)水平,采用羟胺法测定超氧化物歧化酶(SOD)活性,采用硫代巴比妥酸法测定丙二醛(MDA)水平。采用免疫组化方法检测胎盘组织中AGE和RAGE蛋白的表达水平。采用逆转录PCR技术检测两组胎盘组织中RAGE mRNA表达水平。采用免疫印迹法检测两组胎盘组织中RAGE蛋白表达水平。结果GDM组孕妇孕24~26及37~39周血清中AGE、MDA水平均显著高于对照组(t=5.99~14.18,P0.05),而sRAGE、SOD水平均明显低于对照组(t=6.15~10.85,P0.05);GDM组孕24~26及37~39周血清AGE水平与MDA水平均呈正相关(r=0.454、0.489,P0.05),与SOD水平均呈负相关(r=-0.544、-0.422,P0.05)。GDM组孕妇孕24~26及37~39周血清中AGE、sRAGE、SOD、MDA水平比较差异无显著性(P0.05)。GDM组孕妇胎盘裂解液中AGE、MDA水平均显著高于对照组(t=6.84、13.13,P0.05);而SOD水平明显低于对照组(t=10.86,P0.05)。GDM组孕妇胎盘裂解液中AGE水平与MDA水平呈明显正相关(r=0.472,P0.05),与SOD水平呈负相关(r=-0.568,P0.05)。免疫组化染色结果显示,AGE和RAGE蛋白均表达于胎盘合体滋养细胞、血管内皮细胞和巨噬细胞中。GDM组孕妇胎盘组织中RAGE mRNA及蛋白的表达水平显著高于对照组(t=5.538、2.818,P0.01)。结论 AGEs及RAGE可能与GDM孕妇的氧化应激反应有关。
[Abstract]:Objective to investigate the correlation between the expression level of advanced glycosylated end products (AGEs) and AGE receptor (RAGE) in the serum and placental tissues of pregnant women with gestational diabetes (GDM) and the correlation between the expression of AGE receptor (RAGE) and oxidative stress. Methods 80 cases of GDM pregnant women (group GDM) were selected from March 2013 to May 2014 in our obstetrics department and were given childbirth (group GDM), and the same period was selected for the same period. 80 normal pregnant women with similar BMI in age, pregnancy and pre pregnancy were used as control group. The levels of AGE, soluble RAGE (sRAGE) in serum and placental tissue lysates in two groups of pregnant women were detected by enzyme linked immunosorbent assay (ELISA), and the activity of superoxide dismutase (SOD) was determined by hydroxylamine method and thiobarbituric acid method was used. The level of malondialdehyde (MDA) was measured. The expression level of AGE and RAGE protein in placenta tissue was detected by immunohistochemical method. The expression level of RAGE mRNA in two groups of placental tissues was detected by reverse transcription PCR. The expression of RAGE protein in the two groups of placental tissues was detected by immunoblotting. AGE in the serum of pregnant GDM group pregnant women pregnant 24~26 and 37~39 weeks, M The level of DA was significantly higher than that of the control group (t=5.99~14.18, P0.05), while the level of sRAGE and SOD was significantly lower than that of the control group (t=6.15~10.85, P0.05), and the level of 24~26 and 37~39 Zhou Xueqing AGE in GDM group was positively correlated with the MDA level. There was no significant difference in the level of sRAGE, SOD and MDA (P0.05) in.GDM group, the level of AGE in the placental lysate of pregnant women was significantly higher than that of the control group (t=6.84,13.13, P0.05), while the SOD level was significantly lower than that of the control group (t=10.86, P0.05). R=-0.568, P0.05). The results of immunohistochemical staining showed that both AGE and RAGE protein were expressed in the placental syncytial trophoblast cells, and the expression level of RAGE mRNA and protein in the placental tissue of.GDM group of vascular endothelial cells and macrophages was significantly higher than that of the control group (t=5.538,2.818, P0.01). Conclusion AGEs and RAGE may be associated with the oxidative stress reaction of pregnant women. Of
【作者单位】：青岛大学附属医院产科;
【分类号】：R714.256

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