不同浓度米非司酮抑制子宫内膜癌细胞增殖的作用研究

发布时间：2018-05-22 19:26

本文选题：人子宫内膜细胞株 + 米非司酮　；参考：《中国临床药理学杂志》2015年15期

【摘要】：目的探讨不同浓度米非司酮对人子宫内膜癌HEC-1-B细胞株的增殖抑制作用。方法对人子宫内膜癌HEC-1-B细胞株进行体外培养,细胞呈指数增长期时用不同浓度(0,0.1,1,10和100μmol·L-1)的米非司酮对细胞株进行处理,并继续培养观察96 h,用四甲基偶氮唑蓝比色法检测不同浓度米非司酮作用下,不同时点细胞增殖能力。用免疫印迹法检测0,1,10μmol·L-1米非司酮作用48 h后Ki-67和C-myc蛋白表达水平。结果不同浓度的米非司酮对人子宫内膜癌HEC-1-B细胞株均有一定的增殖抑制作用,且随浓度的增加,作用时间的延长,抑制作用明显增强(P0.05)。不同浓度米非司酮可抑制Ki-67和C-myc蛋白表达,且成剂量依赖性(P0.05)。结论米非司酮可能通过Ki-67和C-myc通路抑制人子宫内膜癌HEC-1-B细胞株的增殖作用。
[Abstract]:Objective to investigate the inhibitory effect of mifepristone on the proliferation of human endometrial carcinoma cell line HEC-1-B. Methods Human endometrial carcinoma HEC-1-B cell lines were cultured in vitro. The cells were treated with Mifepristone with different concentrations of 0. 1 渭 mol L ~ (-1) and 100 渭 mol L ~ (-1) during the exponential growth period. After 96 h of culture, the proliferation ability of the cells was detected by tetramethylazolium blue colorimetry under different concentrations of mifepristone. The expression of Ki-67 and C-myc protein was detected by Western blot after exposure to 10 渭 mol L-1 mifepristone for 48 h. Results Mifepristone at different concentrations could inhibit the proliferation of human endometrial carcinoma HEC-1-B cell line to a certain extent. With the increase of concentration and the prolongation of action time, the inhibitory effect of mifepristone on human endometrial carcinoma HEC-1-B cell line was obviously enhanced. Mifepristone could inhibit the expression of Ki-67 and C-myc protein in a dose-dependent manner. Conclusion Mifepristone may inhibit the proliferation of human endometrial carcinoma HEC-1-B cell line through Ki-67 and C-myc pathways.
【作者单位】：福建医科大学附属第一医院妇产科;
【基金】：福建省卫生厅青年科研基金资助项目(200624)
【分类号】：R737.33

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