Furin抑制剂α1-PDX对宫颈癌HeLa细胞生长、转移和成瘤的影响

发布时间：2018-05-24 14:54

  本文选题：宫颈癌 + HeLa　； 参考：《南方医科大学学报》2015年03期

【摘要】：目的研究α1-PDX对宫颈癌细胞株He La细胞生长、转移及成瘤的影响并探讨其机制。方法α1-PDX转染He La细胞,通过MTT细胞增殖实验,Boyden细胞迁移和侵袭实验观察He La细胞的生长,迁移和侵袭能力。采用蛋白印迹法检测细胞Furin及产物膜性Ⅰ型金属蛋白酶(MT1-MMP)蛋白量的变化,荧光酶底物实验检测Furin活性。制作He La细胞致瘤裸鼠模型研究肿瘤体内生长。结果细胞增殖实验结果显示,与对照组相比24 hα1-PDX组He La细胞体外生长抑制18.4%,差异有统计学意义(P0.01);细胞迁移和侵袭实验结果显示,α1-PDX处理组穿过滤膜和基质胶的He La细胞数量均明显少于对照组(P0.01);He La/PDX可显著降低Furin的活性和MTI-MMP的蛋白水平。He La/PDX致瘤裸鼠的成瘤机率及皮下肿瘤体积明显小于对照组。结论α1-PDX可有效抑制人宫颈癌He La细胞的生长、转移及成瘤能力,机制可能与降低Furin活性及产物MTI-MMP的表达有关。
[Abstract]:Objective to study the effect of 伪 1-PDX on the growth, metastasis and tumorigenesis of cervical cancer cell line He-La. Methods He-La cells were transfected with 伪 1-PDX. The growth, migration and invasion of He-La cells were observed by MTT cell proliferation assay. The changes of Furin and MT1-MMPin protein were detected by Western blot, and the activity of Furin was detected by fluorescence enzyme substrate assay. A nude mouse model of helium La cell tumorigenesis was established to study the tumor growth in vivo. Results the results of cell proliferation test showed that, Compared with the control group, the growth inhibition of He-La cells in vitro in 24 h 伪 1-PDX group was significantly lower than that in the control group (P 0.01). The results of cell migration and invasion experiments showed that the number of He-La cells in 伪 1-PDX treatment group was significantly lower than that in control group (P 0.01), and the number of He-La cells in 伪 1-PDX treatment group was significantly lower than that in the control group (P 0.01). La/PDX significantly decreased the activity of Furin and the protein level of MTI-MMP. The probability of tumorigenesis and subcutaneous tumor volume in nude mice induced by he La/PDX were significantly lower than those in the control group. Conclusion 伪 1-PDX can effectively inhibit the growth, metastasis and tumorigenesis of human cervical cancer He-La cells. The mechanism may be related to the decrease of Furin activity and the expression of MTI-MMP.
【作者单位】： 唐山市妇幼保健院生殖遗传科;河北联合大学研究生学院;
【基金】：河北省自然科学基金(H2012401019) 唐山市科学技术研究与发展计划项目(14130246a)
【分类号】：R737.33
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本文编号：1929454