IL-6对人卵巢癌细胞体外增殖及锚定非依赖生长能力的影响

发布时间：2018-05-25 09:16

本文选题：IL- + 细胞增殖　；参考：《免疫学杂志》2015年10期

【摘要】：目的研究内源性IL-6对卵巢癌(ovarian cancer,OVCA)细胞体外增殖及锚定非依赖生长能力的影响并探讨其作用机制。方法应用前期建立的内源性过表达IL-6的人卵巢癌A2780细胞株和内源性抑制IL-6表达的人卵巢癌SKOV-3细胞株,分别采用MTT法、双层软琼脂集落形成实验、流式细胞术、RT-PCR和Western blot技术检测IL-6对OVCA增殖及锚定非依赖生长能力的影响,并对其作用机制进行研究。结果与对照组细胞相比,过表达IL-6的A2780细胞的增殖及锚定非依赖性生长能力增强(P0.01),而IL-6抑制表达的SKOV-3细胞则降低(P0.01)。进一步研究表明IL-6促OVCA细胞增殖作用是通过改变细胞周期分布而非抑制细胞凋亡来实现的;过表达IL-6可显著提高A2780细胞的Cyclin D1、Cyclin B1的表达水平,而抑制IL-6表达则明显降低SKOV-3细胞的Cyclin D1、Cyclin B1的表达水平;应用ERK或Akt特异性信号阻断剂可显著抑制高表达IL-6的A2780细胞增殖及Cyclin D1、Cyclin B1的表达水平。结论 IL-6能促进OVCA细胞的增殖和锚定非依赖性生长,IL-6促OVCA细胞增殖作用是通过增加Cyclin D1、Cyclin B1表达水平从而改变细胞周期分布来实现的,并与活化的PI3K/Akt、Ras/MEK/ERK信号通路有关。
[Abstract]:Objective to investigate the effect of endogenous IL-6 on proliferation and anchoring ability of ovarian cancer OVCA cells in vitro and its mechanism. Methods A human ovarian cancer cell line A2780 with endogenous overexpression of IL-6 and a human ovarian cancer SKOV-3 cell line with endogenous inhibition of IL-6 expression were established by using MTT assay and double layer soft Agar colony forming assay respectively. The effects of IL-6 on the proliferation of OVCA and the ability of anchoring independent growth were detected by flow cytometry and Western blot, and the mechanism was studied. Results compared with the control group, the proliferation and anchoring independent growth ability of A2780 cells with overexpression of IL-6 increased P0.01a, while SKOV-3 cells inhibited by IL-6 decreased P0.01a. Further studies have shown that IL-6 promotes the proliferation of OVCA cells by changing cell cycle distribution rather than inhibiting cell apoptosis, and that overexpression of IL-6 can significantly increase the expression of Cyclin D1 cyclin B1 in A2780 cells. Inhibition of IL-6 expression significantly decreased the expression level of Cyclin D1 cyclin B1 in SKOV-3 cells, and ERK or Akt specific signal blockers could significantly inhibit the proliferation of A2780 cells and the expression of Cyclin D1 cyclin B1 in A2780 cells with high IL-6 expression. Conclusion IL-6 can promote the proliferation of OVCA cells and anchor independent growth of IL-6 to promote the proliferation of OVCA cells by increasing the expression level of Cyclin D1Cyclin B1 and changing the cell cycle distribution, which is related to the activated PI3K / AktRas/ Mek / ERK signaling pathway.
【作者单位】：天津市职业与环境危害生物标志物重点实验室;天津武警后勤学院病原生物学与免疫学教研室;天津武警后勤学院药理学教研室;
【基金】：国家自然科学基金(81041071,81273520,81572852,81502256) 天津市自然科学基金重点项目(12JCZDJC26300) 武警后勤学院科学技术研究项目(WHZ201202,WHB201404,WHB201405,WHB201406,2015ZXKF05,WHB201505,WHJ201508)
【分类号】：R737.31

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