蛋白激酶CK2抑制剂四溴苯三唑可促进卵巢癌CAOV3细胞凋亡

发布时间：2018-05-25 22:57

本文选题：CKα + 四溴苯三唑　；参考：《第三军医大学学报》2015年14期

【摘要】：目的观察蛋白激酶CK2抑制剂四溴苯三唑(4,5,6,7-tetrabromobenzotriazole,TBB)对卵巢癌CAOV3细胞增殖、凋亡及细胞凋亡调控因子Bcl-2、Bax表达的影响,进一步探讨TBB促进卵巢癌CAOV3细胞凋亡的作用机制。方法体外培养CAOV3细胞,将其分为对照组、DMSO组和TBB组(终浓度为12.5、25、50、75μmol/L)。MTT测定各组细胞增殖情况;流式细胞仪检测各组卵巢癌CAOV3细胞凋亡;Western blot检测各组CAOV3细胞CK2α及凋亡相关因子Bcl-2、Bax的表达。结果与对照组[(92.00±4.36)%]比较,TTB各浓度组细胞增殖率分别为(73.00±2.76)%、(67.00±3.51)%、(52.00±2.83)%和(40.00±1.75)%,随着TBB浓度的增加,其对CAOV3细胞增殖的抑制呈剂量依赖性增强(P0.01);细胞凋亡比例也呈剂量依赖性增加;与对照组比较,CK2α的表达逐渐降低,Bcl-2蛋白表达减少,Bax蛋白表达增加(P0.01)。结论 TBB可通过抑制抗凋亡蛋白Bcl-2的表达和促进促凋亡蛋白Bax的表达而促进卵巢癌CAOV3细胞凋亡。
[Abstract]:Objective to investigate the effects of protein kinase CK2 inhibitor tetrabromobenzotriazoletriazole (TBBB) on the proliferation, apoptosis and the expression of apoptosis-regulating factor Bcl-2nBax in ovarian cancer CAOV3 cells, and to explore the mechanism of TBB in promoting apoptosis of ovarian cancer CAOV3 cells. Methods CAOV3 cells were cultured in vitro and divided into control group and TBB group. The expression of CK2 伪 and apoptosis-related factor Bcl-2pBax in CAOV3 cells of each group was detected by flow cytometry and the expression of CK2 伪 in CAOV3 cells was detected by Western blot. Results compared with the control group (92.00 卤4.36%), the cell proliferation rates of each concentration group were 73.00 卤2.76%, 67.00 卤3.51% and 40.00 卤1.75%, respectively. With the increase of TBB concentration, the inhibition of CAOV3 cell proliferation was enhanced in a dose-dependent manner, and the percentage of apoptosis was also increased in a dose-dependent manner. Compared with the control group, the expression of CK2 伪 decreased gradually, the expression of Bcl 2 protein decreased and the expression of Bax protein increased (P 0.01). Conclusion TBB can promote the apoptosis of ovarian cancer CAOV3 cells by inhibiting the expression of anti-apoptotic protein Bcl-2 and promoting the expression of pro-apoptotic protein Bax.
【作者单位】：辽宁医学院生物化学与分子生物学教研室;
【基金】：辽宁省科学技术计划项目(2013225305)~~
【分类号】：R737.31

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