SB203580对氧化应激下人绒毛外滋养细胞生物学功能的影响

发布时间：2018-05-26 14:02

本文选题：p丝裂原活化蛋白激酶 + SB　；参考：《上海交通大学学报(医学版)》2017年02期

【摘要】：目的·探讨p38丝裂原活化蛋白激酶(p38 MAPK)抑制剂SB203580对缺氧/复氧条件下人绒毛外滋养细胞生物学功能的影响。方法·选取体外培养的早孕绒毛外植体和人绒毛外滋养细胞株HTR8/SVneo,根据干预方式的不同各分为4组:对照组、SB203580组(阻断p38 MAPK信号通路)、缺氧/复氧组(氧化应激模型体外模拟子痫前期)和SB203580+缺氧/复氧组。观察SB203580对氧化应激下早孕绒毛外植体中绒毛外滋养细胞生物学功能的影响。Western blotting检测HTR8/SVneo中p38 MAPK蛋白表达及磷酸化水平,Transwell实验观察细胞侵袭和迁移能力,明胶酶谱法和ELISA法分别检测细胞培养上清液中基质金属蛋白酶2/9(MMP2/9)的活性及可溶性fms样酪氨酸激酶-1(s Flt-1)和可溶性内皮因子(s Eng)的水平。结果·SB203580可促进氧化应激下早孕绒毛外植体中绒毛外滋养细胞的外生性迁移。缺氧/复氧可使HTR8/SVneo细胞的迁移和侵袭能力下降,p38 MAPK磷酸化水平升高,s Flt-1和s Eng分泌增加;而SB203580可使氧化应激下HTR8/SVneo中p38 MAPK磷酸化水平降低,细胞迁移和侵袭能力提高,细胞培养上清液中MMP2/9活性增加、s Flt-1和s Eng的分泌量减少。结论·SB203580对氧化应激下人绒毛外滋养细胞的生物学功能具有保护作用。
[Abstract]:Objective to investigate the effects of p38 mitogen-activated protein kinase (p38) MAPK inhibitor SB203580 on the biological function of human extracellular trophoblastic cells under hypoxia / reoxygenation. Methods Human trophoblastic cell line HTR8 / SVneowas cultured in vitro and divided into 4 groups: control group SB203580 (blocking p38 MAPK signaling pathway) and hypoxia / reoxygenation group (oxidative stress model). Type A in vitro mimic preeclampsia) and SB203580 hypoxia / reoxygenation group. To observe the effect of SB203580 on the biological function of extracellular trophoblastic cells in early pregnancy under oxidative stress. Western blotting was used to detect the expression of p38 MAPK protein and phosphorylation level in HTR8/SVneo. The activity of matrix metalloproteinase 2 / 9 MMP2 / 9) and the levels of soluble fms like tyrosine kinase-1 (Flt-1) and soluble endothelial factor (SEC) in supernatant of cell culture were detected by gelatin zymogram and ELISA method, respectively. Results SB203580 could promote the exophytic migration of trophoblast in early pregnancy villi explants under oxidative stress. Hypoxia / reoxygenation decreased the migration and invasion ability of HTR8/SVneo cells and increased the phosphorylation level of p38 MAPK and the secretion of s Eng, while SB203580 decreased the phosphorylation level of p38 MAPK and increased the migration and invasion of HTR8/SVneo under oxidative stress. The activity of MMP2/9 in the supernatant of cell culture increased the secretion of s Flt-1 and s Eng. Conclusion SB203580 has protective effect on the biological function of human extracellular trophoblast under oxidative stress.
【作者单位】：重庆市妇幼保健院;重庆医科大学附属第一医院;
【基金】：国家自然科学基金(81300508)~~
【分类号】：R714.244

【相似文献】