基于液相色谱-质谱联用技术的多囊卵巢综合征患者血清脂质组学分析

发布时间：2018-05-26 21:18

本文选题：多囊卵巢综合征 + 液相色谱-质谱联用　；参考：《分析化学》2015年10期

【摘要】：多囊卵巢综合征是育龄期妇女最为常见的内分泌以及代谢紊乱疾病,是女性不孕的主要原因之一。多囊卵巢综合征患者临床存在很大的异质性,其致病机理尚未完全阐明。本研究基于反相液相色谱与串联四级杆飞行时间质谱联用技术(RPLC/Q-TOF-MS),对74例多囊卵巢综合征患者和54例正常女性的血清进行了脂质组学分析,定性鉴别出15类314种脂质化合物。数据采用非参数检验寻找潜在的差异化合物,p0.05为显著性差异。结果表明:多囊卵巢综合征患者与正常人相比,不饱和脂肪酸、部分鞘磷脂、甘油二酯、甘油三酯呈上调趋势;部分磷脂酰胆碱、溶血性磷脂酰胆碱、溶血性磷脂酰乙醇胺呈下调趋势。而肥胖多囊卵巢综合征患者与非肥胖多囊卵巢综合征患者相比,不饱和脂肪酸、鞘磷脂、甘油二酯、甘油三酯上调更为明显;溶血性磷脂酰胆碱、部分磷脂酰胆碱下调更为明显。本研究结果表明,脂质组学是研究疾病分型的有效手段。
[Abstract]:Polycystic ovary syndrome (PCOS) is the most common endocrine and metabolic disorder in women of childbearing age and is one of the main causes of female infertility. The pathogenesis of polycystic ovary syndrome (PCOS) has not been fully elucidated. Based on the RPLC / Q-TOF-MSM technique of RPLC / Q-TOF-MS, the serum lipids of 74 patients with polycystic ovary syndrome (PCOS) and 54 normal women were analyzed qualitatively, and 314 lipid compounds of 15 classes were identified qualitatively. The nonparametric test was used to search for potential difference compounds (p0.05) as significant difference. The results showed that unsaturated fatty acids, partial sphingomyelin, diglyceride, triglyceride, phosphatidylcholine, lysophosphatidylcholine, and hemolytic phosphatidylcholine increased in the patients with polycystic ovary syndrome. Lysophosphatidyl ethanolamine showed a downward trend. The unsaturated fatty acids, sphingomyelin, diglyceride and triglyceride were up-regulated in obese patients with polycystic ovary syndrome (PCOS). Part of phosphatidylcholine down-regulated more obviously. The results of this study indicate that lipigraphy is an effective method for the study of disease classification.
【作者单位】：中国科学院大连化学物理研究所;黑龙江中医药大学附属第一医院妇产科;
【基金】：国家公益性行业专项基金资助项目(No.201107005)
【分类号】：R711.75;O657.63

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