低分子肝素对不同氧浓度下早孕绒毛滋养层细胞侵袭力的影响作用

发布时间：2018-05-27 13:10

本文选题：低分子肝素 + 早孕绒毛滋养层细胞　；参考：《现代妇产科进展》2017年06期

【摘要】：目的:研究低分子肝素(LMWH)对不同氧浓度下早孕绒毛滋养层细胞侵袭力的影响。方法:取妊娠8~10周正常绒毛组织,酶消化法分离人早孕绒毛滋养层细胞,分别置2%和20%O_2分压的细胞培养箱培养。用不同浓度LMWH(0、0.1、5、10IU/ml)处理滋养细胞,观察滋养层细胞侵袭力的改变,检测细胞中HIF-1α、MMP-2、MMP-9、TIMP-2、TIMP-3表达。结果:低氧浓度下,早孕绒毛滋养层细胞的侵袭能力随着LMWH浓度(0,0.1,5IU/ml)升高而增强,但高浓度的LMWH(10IU/ml)对其有抑制作用;随着LMWH浓度增加,HIF-1α和MMP-2表达呈先上升后下降的趋势;TIMP-2、TIMP-3表达改变的趋势与MMP-2相同。正常氧浓度下的滋养层细胞,其侵袭能力未见显著增强,各细胞因子的表达无上调。结论:低氧环境下,一定浓度的LMWH可提高早孕绒毛滋养层细胞的侵袭能力,其发生机制可能是通过诱导、增强滋养层细胞HIF-1α和MMP-2基因表达。
[Abstract]:Aim: to study the effect of low molecular weight heparin (LMWHs) on the invasion of trophoblast cells in early pregnancy. Methods: normal chorionic villi were isolated from normal chorionic villi at the 8th week of gestation and cultured in 2% and 20%O_2 incubators by enzyme digestion. Trophoblastic cells were treated with different concentrations of LMWH0 / 0.1 and 510U / ml. The changes of invasion of trophoblastic cells were observed and the expression of TIMP-2TIMP-3 in HIF-1 伪 -MMP-2MMP-9 and TIMP-2were detected. Results: the invasion ability of trophoblastic cells in early pregnancy increased with the increase of LMWH concentration of 0. 1 ~ 5 渭 mol / ml, but it was inhibited by high concentration of LMWH-10U / ml. With the increase of LMWH concentration, the expression of HIF-1 伪 and MMP-2 increased first and then decreased. The change of TIMP-2 TIMP-3 expression was the same as that of MMP-2. The invasion ability of trophoblast cells at normal oxygen concentration was not significantly enhanced, and the expression of cytokines was not up-regulated. Conclusion: under hypoxic environment, LMWH at a certain concentration can enhance the invasion ability of trophoblast cells in early pregnancy, and its mechanism may be to enhance the expression of HIF-1 伪 and MMP-2 genes in trophoblast cells through induction.
【作者单位】：山东大学齐鲁医院妇产科;
【基金】：山东省自然科学基金资助项目(No:BS2011YY025)
【分类号】：R714

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