生殖道支原体致不孕患者输卵管Toll样受体表达及意义

发布时间：2018-05-27 16:31

本文选题：支原体 + 输卵管不孕　；参考：《中国现代医学杂志》2015年10期

【摘要】：目的探讨支原体(Mg)感染致输卵管不孕患者组织Toll样受体(TLR)表达及临床意义。方法选择40例输卵管不孕且伴生殖道支原体感染并行手术患者及20例健康对照,采用免疫组织化学方法检测输卵管中Toll样受体2和4的表达,及ELISA法检测输卵管液中肿瘤坏死因子α(TNF-α)及白介素-1(IL-1)的含量,并进行比较。结果 40例支原体感染致不孕者与对照组输卵管TLR2和TLR4均为阳性,支原体感染不孕者输卵管TLR2阳性细胞计数为(13.0±2.9),高于对照组(7.9±1.9);TLR4阳性细胞计数为(27.7±7.7),高于对照组(9.7±2.8),两组间差异均具有统计学意义(P0.01)。支原体感染不孕患者输卵管液IL-1与TNF-α含量均高于对照组(P0.01)。输卵管不孕患者TLR2表达与IL-1和TNF-α表达具有正相关性(r=0.763和0.394,P0.05);TLR4表达与IL-1和TNF-α表达具有正相关性,相关系数(r=0.755和0.538,P0.01)。结论 TLR2和TLR4在支原体所致输卵管不孕患者中表达增加,可能通过影响细胞因子而参与输卵管炎症。
[Abstract]:Objective to investigate the expression and clinical significance of Toll-like receptor in patients with tubal infertility caused by mycoplasma. Methods the expression of Toll like receptor 2 and 4 was detected by immunohistochemical method in 40 patients with tubal infertility and mycoplasma infection of genital tract and 20 healthy controls. And ELISA method was used to detect the contents of TNF- 伪 and IL-1in oviduct fluid. Results both TLR2 and TLR4 of fallopian tubes were positive in 40 cases of infertility caused by mycoplasma infection. The number of TLR2 positive cells in fallopian tube of infertile patients infected with mycoplasma was 13.0 卤2.9m, which was higher than that of control group (7.9 卤1.9) and that of control group was 27.7 卤7.7 and 9.7 卤2.8 respectively. The difference between the two groups was statistically significant (P 0.01). The contents of IL-1 and TNF- 伪 in fallopian tube fluid of infertile patients infected with mycoplasma were higher than those in control group (P 0.01). There was a positive correlation between the expression of TLR2 and the expression of IL-1 and TNF- 伪 in infertile oviducts. There was a positive correlation between the expression of TLR4 and the expression of IL-1 and TNF- 伪. Conclusion the expression of TLR2 and TLR4 in mycoplasma induced tubal infertility is increased, which may be involved in salpingositis by affecting cytokines.
【作者单位】：湖南省妇幼保健院妇科;湖南省人民医院皮肤科;
【基金】：湖南省科技厅计划项目(No:2013FJ3108) 湖南省人民医院仁术科研基金项目(No:2014-5)
【分类号】：R711.6

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