Merlin蛋白在青海汉、藏、回族子宫颈鳞癌中的表达意义

发布时间：2018-05-29 02:41

本文选题：子宫颈鳞癌 + NF2基因　；参考：《青海大学》2017年硕士论文

【摘要】：目的:探索NF2基因蛋白Merlin于青海女性非病变宫颈组织及子宫颈鳞状细胞癌内的显示状况;比较青海汉族、藏族、回族宫颈鳞癌Merlin蛋白的显示是否存在民族差异性,并且对Merlin蛋白在各民族宫颈鳞癌病患的年纪、FIGO期别、淋巴转移情况及细胞分化水平等临床病理特性间的显示情况进行进一步探讨。方法:收集2014年07月至2016年07月因子宫颈鳞癌在青海红十字医院行宫颈癌根治术治疗的108例子宫颈鳞癌组织标本为研究组(SCC),其中汉族40例,藏族38例,回族30例;收集同时期年龄相近的因子宫肌瘤在我院住院治疗的107例正常宫颈组织标本为对照组(NCE),其中汉族40例,藏族37例,回族30例。免疫组化方法被用于检测Merlin蛋白的表达情况;SPSS 20.0软件被应用于进行数据处理分析,χ2检验和Fisher确实概率法验证各组率的差异。结果:1.SCC组Merlin蛋白阳性表达率为17.6%(19/108),NCE组Merlin蛋白阳性表达率为89.7%(96/107),明显比SCC组高(P0.05),二者对比差异显著;其中NCE组40例汉族、37例藏族、30例回族Merlin蛋白阳性表达率分别为90.0%(36/40)、91.9%(34/37)、86.7%(26/30);SCC组40例汉族、38例藏族、30例回族Merlin蛋白阳性表达率分别为17.5%(7/40)、18.4%(7/38)、16.7%(5/30),在各种族中SCC组Merlin蛋白非阴性显示结果比对NCE组都存在统计学差别(P0.05);2.汉、藏、回SCC中Merlin蛋白阳性表达结果经比对无民族差别(P0.05),同样对比汉、藏、回NCE中Merlin蛋白阳性显示结果亦未见民族差别(P0.05);3.SCC组中40例汉族,分别对Merlin蛋白在不同年龄、FIGO分期、淋巴结转移情况及细胞分化程度中的阳性表达率进行了比较,未见在各临床病理参数内Merlin蛋白非阴性显示率存在明显差别(P0.05),同样对SCC组中38例藏族及30例回族中Merlin蛋白在各临床病理参数中的阳性表达进行比较,均无明显差异。结论:1.Merlin蛋白阳性显示于青海女性子宫颈鳞癌中比非病变宫颈上皮显著减少,提示子宫颈鳞癌的发生或许与Merlin蛋白显示降低、缺失密切相关,Merlin蛋白检测有助于辅助推断子宫颈鳞癌发生病因;2.Merlin蛋白于汉、藏、回族非病变宫颈上皮及宫颈鳞状细胞癌内显示无民族差异,提示种族间子宫颈鳞癌发生率的差异可能不受Merlin蛋白表达的影响;3.汉、藏、回三民族中子宫颈鳞癌组织Merlin蛋白的表达均与患者年纪、FIGO分期、淋巴移动状态、细胞分化水平等特性没关系(P0.05),提示Merlin蛋白表达缺少或许只是子宫颈鳞癌形成的先期事件,并未直接参加子宫颈鳞癌的远处移动、粘附扩散等流程。
[Abstract]:Objective: to explore the display status of NF2 gene Merlin in non-pathological cervical tissues and squamous cell carcinoma of cervix in Qinghai women, and to compare whether there are ethnic differences in the display of Merlin protein in cervical squamous cell carcinoma of Qinghai Han nationality, Tibetan nationality and Hui nationality. Furthermore, the display of Merlin protein in the stage of Figo, the status of lymphatic metastasis and the level of cell differentiation in patients with cervical squamous cell carcinoma of different nationalities were further discussed. Methods: from July 2014 to July 2016, 108 cases of cervical squamous cell carcinoma treated by radical cervical cancer in Qinghai Red Cross Hospital were collected as study group (40 cases of Han nationality, 38 cases of Tibetan nationality and 30 cases of Hui nationality). A total of 107 normal cervical tissue specimens of the same age, including 40 Han, 37 Tibetans and 30 Hui, were collected from normal cervix tissues for hysteromyoma in our hospital. Immunohistochemical method was used to detect the expression of Merlin protein. SPSS20.0 software was used for data processing and analysis. 蠂 2 test and Fisher positive probability method were used to verify the difference in the rates of each group. Results 1. The positive expression rate of Merlin protein in SCC group was 17.610 / 10 8% and the positive expression rate of Merlin protein was 89.7 / 96 / 107%, which was significantly higher than that in SCC group (P 0.05). The difference between the two groups was significant. The positive expression rate of Merlin protein in NCE group (40 cases), Han nationality (37 cases), Tibetan group (30 cases), Hui nationality (30 cases) was 90.0%, respectively. The positive expression rate of Merlin protein in SCC group was 17.5% 18.40% 18.440% 18.440% 18.440%, respectively, and the positive rate of Merlin protein in SCC group was 16.7% 30%. The non-negative expression rate of Merlin protein in SCC group was negative in all races. The positive expression rate of Merlin protein in 38 cases of Han nationality and 30 cases of Hui nationality was 17.55% -18.40% 18.44%, respectively. There was statistical difference in NCE group (P 0.05). The results of positive expression of Merlin protein in SCC of Han, Tibetan and Hui were compared with that of no ethnic difference (P0.05). Similarly, the positive results of Merlin protein in NCE of Han and Tibetan were also not found. 3. In the group of Han nationality, 40 cases of Han nationality in the group of NCE were divided into different age and stage of FIGO, respectively. The positive expression rates of lymph node metastasis and cell differentiation were compared. There was no significant difference in the non-negative display rate of Merlin protein among different clinicopathological parameters. There was no significant difference in the positive expression of Merlin protein in 38 cases of Tibetan nationality and 30 cases of Hui nationality in SCC group. Conclusion: 1. The presence of Merlin protein in cervical squamous cell carcinoma in Qinghai women was significantly lower than that in non-pathological cervical epithelium, suggesting that the occurrence of cervical squamous cell carcinoma might be lower than that of Merlin protein. Detection of Merlin protein is closely related to deletion. 2. There is no difference in the presence of Merlin protein in cervix epithelium and squamous cell carcinoma of cervix in Han, Zang and Hui nationality. The results suggest that the difference in incidence of cervical squamous cell carcinoma between ethnic groups may not be affected by the expression of Merlin protein. The expression of Merlin protein in cervical squamous cell carcinoma was correlated with Figo stage and lymphatic movement in Han, Zang and Hui Sanmin ethnic groups. It is suggested that the lack of expression of Merlin protein may be a preevent in the formation of squamous cell carcinoma of the cervix, and it does not directly participate in the process of distant migration, adhesion and diffusion of squamous cell carcinoma of the cervix.
【学位授予单位】：青海大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R737.33

【相似文献】