女方染色体多态对体外受精-胚胎移植妊娠结局的影响

发布时间：2018-05-29 18:08

本文选题：女方染色体多态性 + 体外受精-胚胎移植(IVF-ET)　；参考：《中华生殖与避孕杂志》2017年08期

【摘要】：目的探讨女方染色体多态性对体外受精-胚胎移植(IVF-ET)结局的影响。方法回顾性比较IVF-ET助孕治疗的女方染色体多态(65对,女方多胎组)和正常对照夫妇(160对,正常对照组)的妊娠结局。结果女方多态组与正常对照组患者双原核(2PN)受精率、妊娠率、生化妊娠率均无统计学差异(P0.05);然而多态组1PN受精率(8.48%)及多PN受精率(12.50%)均显著高于正常对照组(3.90%,7.96%)(P0.01),卵裂率(89.21%)显著低于正常对照组(98.36%)(P0.01)。虽然妊娠率比较未见明显差异,但多态组种植率(23.89%)低于正常对照组(35.40%)(P0.05)。将不同的多态类型详细分组与正常对照组对比结果显示:1号、9号、16号染色体异染色质增加组1PN受精率(11.36%)及多PN受精率(17.02%)均显著高于正常对照组(3.90%,7.96%)(P0.01),卵裂率(87.90%)显著低于正常对照组(98.36%,P0.01);D/G组随体柄增长组卵裂率(80.00%)及种植率(17.65%)均低于对照组(98.36%,35.40%)(P0.01,P0.05);而9号染色体臂间倒位[inv(9)]患者各项指标与正常对照组相比无统计学差异。结论女方染色体多态,尤其是1号、9号、16号染色体异染色质增加会导致IVF助孕患者异常受精比例增加以及种植率降低;Inv(9)对IVF临床预后无明显影响,临床可根据患者不同的多态类型个体化给与遗传咨询指导。
[Abstract]:Objective to investigate the effect of female chromosome polymorphism on the outcome of IVF-et in vitro fertilization-embryo transfer. Methods the pregnancy outcomes of 65 pairs of female chromosome polymorphisms (65 pairs) and 160 pairs of normal control couples (normal control group) treated with IVF-ET assisted pregnancy therapy were retrospectively compared. Results the fertilization rate and pregnancy rate of the patients in the female polymorphic group and the normal control group were 2 PNs. There was no significant difference in the biochemical pregnancy rates (P 0.05), but the fertilization rates of 1PN and PN in the polymorphism group were significantly higher than those in the normal control group (P 0.01, P 0.01, P 0.01, P 0.01, P 0.01), and the cleavage rate (89.21) was significantly lower than that in the normal control group (98.36%). Although there was no significant difference in pregnancy rate, the planting rate of polymorphic group was 23.899.It was lower than that of normal control group (35.40%). The results showed that the fertilization rate of 1PN 11.36% and polyPN fertilization rate 17.02% in chromosome 1, 9, 16 heterochromatin increased group were significantly higher than those in normal control group (3.90%, 7.96%, P 0.01, cleavage rate 87.90). In the normal control group, the cleavage rate and implantation rate in the control group were significantly lower than those in the control group (98.36 ~ 35.40 ~ 35.40 ~ 35.40 ~ 35.40 ~ 35.40 ~ 35.40 ~ 35.40%), while there was no significant difference between the patients with chromosome 9 / arm inversion and normal control group. Conclusion the increase of chromosome polymorphisms, especially the heterochromatin of chromosome 1, 9 and 16, will result in the increase of abnormal fertilization rate and the decrease of implantation rate in IVF assisted pregnancy patients. It has no significant effect on the clinical prognosis of IVF. Genetic counseling can be given clinically according to the individual type of polymorphism of the patient.
【作者单位】：武汉大学人民医院生殖医学中心湖北省辅助生育与胚胎发育医学临床研究中心;
【基金】：中央高校青年教师资助项目(2042016kf0086) 默克雪兰诺生殖医学基金(Merck Serono_CREATE-2016141) 国家自然科学基金资助项目(81701412)~~
【分类号】：R714.8

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