孕鼠铁缺乏对子代GH-IGF-1轴的影响

发布时间：2018-05-30 00:13

本文选题：铁缺乏 + 生长激素　；参考：《中国现代医学杂志》2017年08期

【摘要】：目的探讨大鼠妊娠期铁缺乏对新生仔鼠生长激素-胰岛素样生长因子-1(GH-IGF-1)轴的影响及机制。方法 4周龄雌性Wistar大鼠36只,随机分为实验组和对照组,分别饲低铁饲料和含铁饲料喂养4周,检测雌鼠血红蛋白(Hb)浓度,血细胞比容(HCT)、红细胞平均容积(MCV)、红细胞平均血红蛋白量(MCH)、红细胞平均血红蛋白浓度(MCHC)及红细胞分布宽度(RDW)等血液学相关参数。将雌鼠与正常雄鼠合笼,孕末期检测血液学相关参数并行骨髓铁染色。观察仔鼠存活率及体重。实时荧光定量聚合酶链式反应(qRTPCR)方法检测新生仔鼠垂体生长激素(GH)及肝脏胰岛素样生长因子-1(IGF-1)表达量。结果相比对照组,实验组低铁干预4周后孕鼠孕末期Hb、HCT、MCV、MCH及MCHC等水平降低(P0.05),RDW水平升高(P0.05);实验组大鼠骨髓细胞内铁及外铁减少;实验组新生仔鼠体重及仔鼠存活率低于对照组(P0.05);实验组垂体GH信使核糖核酸(mRNA)及肝脏IGF-1 mRNA的表达低于对照组(P0.05)。结论妊娠期铁缺乏下调子代GH和IGF-1 mRNA表达造成子代低出生体重。
[Abstract]:Objective to investigate the effect and mechanism of iron deficiency during pregnancy on growth hormone-insulin-like growth factor-1 (GH-IGF-1) axis in newborn rats. Methods Thirty-six 4-week-old female Wistar rats were randomly divided into experimental group and control group. Hematological parameters such as HCT, mean volume of red blood cell (MCV), mean hemoglobin content (MCHN), mean hemoglobin concentration (MCHCC) and distribution width of red blood cell (RDW) were measured. Bone marrow iron staining was performed at the end of pregnancy to detect the hematologic parameters of female mice and normal male mice. The survival rate and body weight were observed. The expression of growth hormone (GH) and insulin-like growth factor-1 (IGF-1) in liver of newborn rats were detected by real-time fluorescence quantitative polymerase chain reaction (QRT PCR). Results compared with the control group, the levels of HCT, MCV, MCH and MCHC in the late pregnant rats of the experimental group were decreased after the intervention of low iron for 4 weeks, and the levels of RDW in the bone marrow cells of the experimental group were increased, and the levels of iron and external iron in the bone marrow cells of the experimental group were decreased. The body weight and survival rate of newborn rats in the experimental group were lower than those in the control group (P 0.05), and the expression of GH mRNA mRNAs and IGF-1 mRNA in the liver in the experimental group were lower than those in the control group (P 0.05). Conclusion Iron deficiency during pregnancy can decrease the expression of GH and IGF-1 mRNA in offspring and result in low birth weight.
【作者单位】：石河子大学医学院;江苏省涟水县人民医院;
【分类号】：R714.254

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