凋亡抑制因子5在卵巢癌中的表达及临床意义

发布时间：2018-05-31 03:16

本文选题：卵巢癌 + 凋亡抑制因子　；参考：《解剖学报》2017年03期

【摘要】：目的检测凋亡抑制因子5(API5)在卵巢癌中的表达,分析其与临床病理特征的关系。方法Western blotting方法检测2例正常卵巢组织及6例不同级别的卵巢癌组织的表达,组织化学方法检测10例正常卵巢、119例卵巢癌(浆液性腺癌61例、黏液性癌5例、内膜样癌10例、透明细胞癌10例及未分化癌33例)组织中API5的表达,分析其表达与临床病理参数及5年生存率之间的相关性,细胞转染及凋亡流式细胞分析检测API5及顺铂对卵巢癌细胞的作用。结果 API5在卵巢癌组织中的表达高于正常卵巢组织,API5的表达与临床病例特征之间密切相关,Kaplan-Meier生存曲线揭示API5表达高的卵巢癌患者较低表达患者生存率低,流式细胞术分析显示,抑制API5的表达能增加顺铂对卵巢癌细胞的敏感性。结论 API5的异常表达与卵巢癌的发生密切相关,提示API5在预后评价中具有潜在的临床应用价值,有可能成为卵巢癌治疗的潜在靶点。
[Abstract]:Objective to investigate the expression of apoptosis inhibitor 5 (API5) in ovarian cancer and its relationship with clinicopathological features. Methods Western blotting method was used to detect the expression of ovarian carcinoma in 2 normal ovarian tissues and 6 ovarian cancer tissues of different grades. Histochemical method was used to detect the expression of ovarian carcinoma in 119 cases of normal ovarian carcinoma (61 cases of serous adenocarcinoma, 5 cases of mucinous carcinoma, 10 cases of endometrial carcinoma), and 5 cases of serous adenocarcinoma, 5 cases of mucinous carcinoma and 10 cases of endometrial carcinoma. The expression of API5 in 10 cases of clear cell carcinoma and 33 cases of undifferentiated carcinoma was analyzed. The correlation between the expression of API5 and clinicopathological parameters and 5-year survival rate was analyzed. The effect of API5 and cisplatin on ovarian cancer cells was detected by cell transfection and apoptosis flow cytometry. Results the expression of API5 in ovarian cancer tissues was higher than that in normal ovarian tissues. Kaplan-Meier survival curve showed that the survival rate of ovarian cancer patients with high API5 expression was lower than that of ovarian cancer patients with high API5 expression. Inhibition of API5 expression increased the sensitivity of cisplatin to ovarian cancer cells. Conclusion the abnormal expression of API5 is closely related to the occurrence of ovarian cancer, suggesting that API5 has potential clinical value in prognosis evaluation and may be a potential target for the treatment of ovarian cancer.
【作者单位】：南通市如东县人民医院妇产科;南通大学附属医院分院妇科;
【分类号】：R737.31

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