异柠檬酸在精子冻融中保护作用的初探

发布时间：2018-05-31 13:11

本文选题：异柠檬酸 + 前向运动精子　；参考：《南京医科大学学报(自然科学版)》2017年02期

【摘要】：目的:探讨异柠檬酸在精子冻融中的保护作用和机制。方法:选取16例健康生育男性的精液样本,分析精液常规参数后,将每份精液1式5份,1∶1加入冷冻保护剂后混匀,不含异柠檬酸的为阴性对照组,实验组分别添加1.25、2.50、3.75、5.00 mmol/L浓度的异柠檬酸,冷冻复苏后进行精液常规分析。同时另选取9例健康生育男性的精液样本,采用荧光染料5,5′,6,6′四氯-1和1′,3,3′四乙基苯并咪唑基羰化青碘(5,5′,6,6′-tetrachloro-1,1′,3,3'′-tetraethyl-imidacarbo-cyanine iodide,JC-1)单标法检测复苏后精子细胞的线粒体膜电位。结果:复苏后G3组前向运动精子百分率(PR)升高(P0.01),G2组PR升高(P0.05),G3组精子浓度上升(P0.05);G3组精子复苏后线粒体膜电位较对照组增大(P0.05)。结论:精液冷冻保护剂中添加适宜浓度的异柠檬酸可以保护冷冻复苏后精子线粒体功能,提高复苏后精子活力和浓度。
[Abstract]:Objective: to investigate the protective effect and mechanism of isocitric acid on spermatozoa freezing and thawing. Methods: the semen samples of 16 healthy fertile men were collected, and the semen parameters were analyzed. The semen of each semen was mixed in 1 form 5 parts 1: 1 with freezing protectant, and the control group without isocitric acid was used as the negative control group. In the experimental group, the concentration of isocitric acid at 1.25 ~ 2.50 ~ 3.75 ~ 5.00 mmol/L was added respectively, and semen routine analysis was made after freezing and resuscitation. At the same time, semen samples were taken from 9 healthy fertile men. The mitochondrial membrane potential of resuscitated spermatozoa cells was determined by the single standard method of fluorescence dye 5, 5, 5, 6 '-tetraethyl-imidacarbo-cyanine iodide JC-1, and the single standard method of fluorescence dye 5, 5' -3 '-tetraethyl-imidacarbo-cyanine iodide JC-1, which was used to detect the mitochondrial membrane potential of resuscitated spermatozoa cells. Results: after resuscitation, the percentage of forward motility spermatozoa (PRP) in G3 group was higher than that in P0.01G 2 group. The increase of sperm concentration in P0.05 + G3 group was higher than that in P0.05 + G3 group after resuscitation, and the mitochondrial membrane potential in G3 group was higher than that in control group. Conclusion: the proper concentration of isocitric acid in semen cryopreservation protective agent can protect sperm mitochondria function after cryopreservation and improve sperm motility and concentration after resuscitation.
【作者单位】：南京医科大学第一附属医院泌尿外科;南京医科大学附属南京医院泌尿外科;
【分类号】：R392.69

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