miR-26b在卵巢癌组织中的表达与功能

发布时间：2018-06-03 02:14

本文选题：卵巢癌 + miR-b　；参考：《中国老年学杂志》2017年01期

【摘要】：目的探讨microRNA-26b(miR-26b)在卵巢癌组织中的表达情况,并探讨其对卵巢癌细胞株HO8910恶性行为的影响。方法采用实时定量PCR(q PCR)检测72例卵巢癌患者经手术切除的上皮性卵巢癌组织标本(卵巢癌组)中的miR-26b水平,同时选取26例正常卵巢上皮组织(正常组)和45例良性卵巢上皮囊肿组织(良性组)作对照,分析miR-26b表达与临床病理参数(年龄、临床分期、分化程度、组织类型及淋巴结转移)的关系。将化学合成miR-26b前体分子mimics和无关序列转染卵巢癌细胞株HO8910,采用MTT法检测转染24、48、72、96 h后的细胞增殖情况,采用流式细胞仪PI/Annexin V-FITC双染法及PI单染法检测转染48 h后的细胞凋亡及细胞周期情况。结果卵巢癌组的miR-26b水平均低于正常组和良性组(P0.05),且其表达与临床分期、分化程度及淋巴结转移均有关(P0.05)。与对照组相比,过表达组(转染miR-26b mimics)的增殖抑制率和凋亡率均升高,且细胞周期中G0/G1期细胞比例升高而S期细胞比例降低(P0.05)。结论在卵巢癌细胞中上调miR-26b表达可抑制增殖并诱导凋亡及细胞周期组织,在卵巢癌防治中有一定价值。
[Abstract]:Objective to investigate the expression of microRNA-26bmmiR-26b in ovarian carcinoma and its effect on malignant behavior of ovarian cancer cell line HO8910. Methods Real-time quantitative PCR(q was used to detect the level of miR-26b in 72 cases of epithelial ovarian cancer tissues (ovarian cancer group), which were surgically resected. At the same time, 26 normal ovarian epithelial tissues (normal group) and 45 benign ovarian epithelial cyst tissues (benign group) were selected as controls. The expression of miR-26b and clinicopathologic parameters (age, clinical stage, differentiation degree) were analyzed. The relationship between histological type and lymph node metastasis. Chemically synthesized miR-26b precursor molecule mimics and unrelated sequence were transfected into ovarian cancer cell line HO8910. The proliferation of ovarian cancer cell line HO8910 was detected by MTT assay. Apoptosis and cell cycle were detected by flow cytometry PI/Annexin V-FITC double staining and Pi single staining after 48 h transfection. Results the level of miR-26b in ovarian cancer group was lower than that in normal group and benign group, and its expression was related to clinical stage, differentiation degree and lymph node metastasis. Compared with the control group, the proliferation inhibition rate and apoptosis rate of the overexpression group (transfected with miR-26b mimics) increased, and the proportion of G0/G1 phase cells increased and the S phase cell proportion decreased (P0.05%). Conclusion Up-regulation of miR-26b expression in ovarian cancer cells can inhibit proliferation and induce apoptosis and cell cycle tissue, which has a certain value in the prevention and treatment of ovarian cancer.
【作者单位】：抚州市妇幼保健院;江西省妇幼保健院;南京医科大学附属南京市妇幼保健院医学研究中心;
【基金】：国家自然科学基金(81302304)
【分类号】：R737.31

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