ROS对子宫内膜崩解的作用

发布时间：2018-06-03 20:48

本文选题：子宫内膜 + 崩解　；参考：《动物医学进展》2015年03期

【摘要】：为探讨ROS在小鼠月经样模型中的作用,将雌性C57小鼠去势,序惯性的给予雌二醇(E2)或孕酮(P4),模拟增殖期和分泌期,花生油注射至宫腔以人工诱导蜕膜化,将孕酮皮埋管移除以造成孕酮的撤退,建立小鼠月经样模型,并在孕酮撤退前1、3、7h给予抗氧化剂NAC消除ROS,利用大体变化、阴道细胞学涂片和组织形态学方法检测子宫的状态,并利用氮蓝四唑(NBT)染色的组织超氧阴离子色度法检测ROS的含量,以研究ROS在月经发生过程中对子宫内膜崩解的作用。阴道细胞学涂片显示,350 mg/kg和500mg/kg NAC能够抑制子宫内膜的出血,而200mg/kg NAC则不能,子宫大体和组织形态学结果显示,350mg/kg和500mg/kg NAC能够抑制子宫内膜的崩解,而200mg/kg NAC则不能,同时,350mg/kg和500mg/kg NAC能够显著抑制ROS的产生,而200mg/kg NAC则没有。说明350mg/kg是抑制子宫内膜崩解的合适剂量,且ROS对子宫内膜的崩解起至关重要的作用。
[Abstract]:In order to investigate the role of ROS in the menstrual model of mice, female C57 mice were castrated with estradiol E2) or progesterone P4, and then injected with peanut oil into uterine cavity to induce decidualization. The progesterone skin tube was removed to make progesterone retreat, and the menstrual model of mice was established. The mouse menstrual model was established. The rosy was eliminated by the antioxidant NAC 1 h before the withdrawal of progesterone. The state of the uterus was detected by gross changes, vaginal cytological smears and histomorphology. The content of ROS was detected by the method of tissue superoxide anion colorimetry with nitroblue tetrazolium (NBT) staining to study the role of ROS in endometrial disintegration during menstruation. Vaginal cytological smears showed that 500mg/kg NAC and 500mg/kg NAC inhibited endometrial bleeding, whereas 200mg/kg NAC did not. Gross and histomorphologic results showed that 350mg / kg and 500mg/kg NAC could inhibit endometrial disintegration, whereas 200mg/kg NAC could not. At the same time, 350 mg / kg and 500mg/kg NAC significantly inhibited the production of ROS, whereas 200mg/kg NAC did not. The results suggest that 350mg/kg is an appropriate dose for inhibiting endometrial disintegration and ROS plays an important role in endometrial disintegration.
【作者单位】：广西医科大学;山东大学附属济南市中心医院;国家人口计生委科学技术研究所;北京协和医学院研究生院;
【基金】：广西壮族自治区教育厅高校科研项目(YB2014079) 广西医科大学青年科学基金项目(GXMUYSF201318)
【分类号】：R711

【共引文献】