Ghrelin对妊娠小鼠子宫内膜蜕膜化的影响

发布时间：2018-06-04 23:03

本文选题：Ghrelin + 生长激素促分泌素受体　；参考：《广东医学》2015年06期

【摘要】：目的探讨Ghrelin对妊娠小鼠子宫内膜蜕膜化的影响。方法实验将子宫基质细胞分为3组,每组6孔:(1)对照组(只加培养基);(2)Ghrelin组(细胞培养液中加入100 ng/m L重组Ghrelin细胞因子);(3)Ghrelin抗体组(细胞培养液中加入30μg/m L Ghrelin抗体)。通过RT-PCR和免疫组织化学技术,观察Ghrelin及其受体在妊娠小鼠子宫内膜蜕膜化过程中的表达变化;通过子宫基质细胞的分离、培养及宫角注射技术,观察Ghrelin在离体及在体状态下对妊娠小鼠子宫内膜蜕膜化的影响。结果 (1)RT-PCR显示:随着妊娠期和蜕膜化的进展,Ghrelin mRNA表达水平明显升高,至妊娠第8天,其表达水平达到最高。(2)在妊娠第5天,GHS-R蛋白主要表达于腔上皮、腺上皮及胚胎植入位点的蜕膜。至妊娠第6天,其表达水平进一步升高,主要表达于胚胎周围的蜕膜。至妊娠第8天,蜕膜中GHS-R蛋白的表达水平渐进下降。(3)与对照组相比,Ghrelin组子宫基质细胞Hox A10蛋白的表达水平明显升高(P0.01),而Ghrelin抗体组子宫基质细胞Hox A10蛋白的表达水平明显下降(P0.01)。(4)与对照组相比,重组Ghrelin对胚胎植入位点没有明显的影响(P0.05),却明显增加的子宫重量(P0.05);对于Ghrelin抗体组,能明显抑制胚胎植入位点,且妊娠子宫重量的明显下降(P0.01)。结论Ghrelin具有明显促进妊娠小鼠子宫内膜蜕膜化的作用。
[Abstract]:Objective to investigate the effect of Ghrelin on decidualization of endometrium in pregnant mice. Methods uterine stromal cells were divided into 3 groups, each group with 6 holes in each group. The control group was treated with 100 ng/m / L recombinant Ghrelin cytokine 3 hrelin antibody (30 渭 g / mL Ghrelin antibody was added to the cell culture medium). The expression of Ghrelin and its receptors in decidua of pregnant mice was observed by RT-PCR and immunohistochemistry, and the uterine stromal cells were isolated, cultured and injected into uterine horn. To observe the effect of Ghrelin on decidualization of endometrium of pregnant mice in vitro and in vivo. Results RT-PCR showed that the expression of ghrelin mRNA increased significantly during pregnancy and decidualization, and reached the highest level on the 8th day of gestation.) on the 5th day of pregnancy, ghrelin R protein was mainly expressed in the luminal epithelium. Decidua of glandular epithelium and embryo implantation site. On the 6th day of gestation, the expression level increased further, mainly in the decidua around the embryo. Until the eighth day of pregnancy, Compared with the control group, the expression of Hox A10 protein in uterine stromal cells increased significantly in ghrelin group, while the expression level of Hox A10 protein in uterine stromal cells in Ghrelin antibody group decreased significantly compared with that in control group. Recombinant Ghrelin had no obvious effect on embryo implantation site, but significantly increased uterine weight (P0.05N). In Ghrelin antibody group, the embryo implantation site was inhibited and the weight of pregnancy uterus decreased significantly (P0.01). Conclusion Ghrelin can obviously promote decidualization of endometrium in pregnant mice.
【作者单位】：江西省人民医院妇产科;
【基金】：江西省卫生厅科技计划项目(编号:20141018)
【分类号】：R714

【参考文献】