HIF-1α和miR-210在宫颈鳞癌中的表达及其相关性

发布时间：2018-06-05 04:13

本文选题：宫颈癌 + 缺氧诱导因子-α　；参考：《重庆医科大学学报》2015年04期

【摘要】：目的:探讨宫颈癌组织中缺氧诱导因子-1α(hypoxia induced factor-1α)和Micro RNA 210(mi R-210)之间的关系,为宫颈癌的早期发现寻找参考指标。方法:采用免疫组化En Vision法和real-time PCR分别检测52例宫颈鳞癌及20例正常宫颈上皮组织中HIF-1α和mi R-210的表达情况,用Spearman秩相关检验分析癌组织中HIF-1α和mi R-210之间的相关性。结果:宫颈鳞癌组织细胞大部分胞核表达HIF-1α,少部分胞核和胞浆同时表达,HIF-1α在宫颈鳞癌组织中的表达率为73.1%(38/52),正常宫颈组织细胞中无表达。mi R-210在宫颈癌组织中的表达水平较正常宫颈组织显著上调,正常宫颈组织mi R-210的表达水平为1.16±0.76,宫颈鳞癌组织中其表达水平为5.04±1.28(P=0.000);在52例宫颈癌组织中,HIF-1α蛋白表达水平与mi R-210表达呈正相关(r=0.43,P=0.001)。HIF-1α蛋白在正常宫颈组织中均无阳性表达,与mi R-210无相关性。在所有72例样本中进行相关性分析,发现两者的表达呈正相关(r=0.67,P=0.000)。说明随着HIF-1α蛋白表达的增加,mi R-210则随着增加。结论:HIF-1α和mi R-210在宫颈鳞癌组织中均呈高表达,且两者有一定的相关性。推测HIF-1α和mi R-210与宫颈癌的发生发展相关,有助于进一步揭示宫颈癌的发病机制。
[Abstract]:Objective: to investigate the relationship between hypoxia inducible factor-1 伪 hypoxia induced factor-1 伪 and Micro RNA 210(mi R-210 in cervical carcinoma, and to find a reference index for early detection of cervical cancer. Methods: the expression of HIF-1 伪 and miR-210 in 52 cases of cervical squamous cell carcinoma and 20 cases of normal cervical epithelium were detected by immunohistochemical en Vision method and real-time PCR, respectively. The correlation between HIF-1 伪 and miR-210 was analyzed by Spearman rank correlation test. Results: most of the cells of cervical squamous cell carcinoma expressed HIF-1 伪, and a few of them expressed HIF-1 伪 simultaneously in cervical squamous cell carcinoma. The expression rate of HIF-1 伪 in cervical squamous cell carcinoma was 73.1 / 52%, and no expression of .mi R-210 was found in normal cervical tissue. Compared with normal cervix tissue, the level was significantly up-regulated. The expression level of miR-210 was 1.16 卤0.76 in normal cervix and 5.04 卤1.28 in squamous cell carcinoma of cervix, and there was no positive expression of HIF-1 伪 protein in normal cervical tissues. There was no correlation with mi R-210. The correlation analysis of all 72 samples showed that there was a positive correlation between them. The results showed that with the increase of HIF-1 伪 protein expression, MMI R-210 increased. Conclusion both of them are highly expressed in squamous cell carcinoma of the cervix, and there is a certain correlation between them. It is inferred that HIF-1 伪 and miR-210 are related to the occurrence and development of cervical cancer, which is helpful to further reveal the pathogenesis of cervical cancer.
【作者单位】：重庆医科大学附属第一医院妇产科;
【基金】：重庆市卫生局科研项目(编号:2013-2-011)
【分类号】：R737.33

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