胡桃醌对宫颈癌SiHa细胞增殖及细胞周期的影响

发布时间：2018-06-05 06:57

本文选题：胡桃醌 + 宫颈肿瘤　；参考：《吉林大学学报(医学版)》2015年04期

【摘要】：目的:探讨胡桃醌对人宫颈癌SiHa细胞增殖及细胞周期的影响,阐明胡桃醌在宫颈癌治疗中的作用。方法:选取处于对数生长期的SiHa细胞,随机分为对照组和10、20、40、60、80和100μmol·L-1胡桃醌处理组。倒置显微镜下观察细胞的形态学变化,噻唑蓝(MTT)法检测细胞增殖活性和细胞生长抑制率,流式细胞术检测SiHa细胞的细胞周期。结果:与对照组比较,胡桃醌处理组SiHa细胞体积缩小,连接消失,与周围细胞脱离。与对照组比较,胡桃醌处理组细胞增殖活性降低(P0.05),细胞生长抑制率升高(P0.05),且呈剂量依赖性,半数抑制浓度(IC50)为20.7μmol·L-1;流式细胞术检测,与对照组比较,10和100μmol·L-1胡桃醌处理组SiHa细胞的亚二倍体峰(SubG1)比率增加,G0/G1和S期细胞比率降低,G2/M期细胞比率先升高后降低。结论:胡桃醌对SiHa细胞增殖具有抑制作用,并可使细胞周期SubG1比率增加。
[Abstract]:Aim: to investigate the effects of nutquinone on the proliferation and cell cycle of human cervical cancer SiHa cells and to elucidate the role of nutcrone in the treatment of cervical cancer. Methods: SiHa cells in logarithmic growth phase were randomly divided into two groups: control group and control group. The morphological changes of the cells were observed under inverted microscope. The cell proliferation activity and cell growth inhibition rate were detected by MTT assay. The cell cycle of SiHa cells was detected by flow cytometry. Results: compared with the control group, the volume of SiHa cells in the treated group was reduced, the connection disappeared, and the cells were separated from the surrounding cells. Compared with the control group, the proliferation activity of the treated group decreased P0.05N, the cell growth inhibition rate increased in a dose-dependent manner, the IC50 was 20.7 渭 mol L-1, and the cell growth inhibition rate was detected by flow cytometry. Compared with the control group, the ratio of hypodiploid subG _ 1) in SiHa cells treated with 10 渭 mol ~ (-1) and 100 渭 mol L ~ (-1) of pectin was increased, and the ratio of G _ (0) / G _ (1) and S phase cells decreased. The cell ratio of G _ 2 / M phase increased at first and then decreased. Conclusion: walnut quinone can inhibit the proliferation of SiHa cells and increase the SubG1 ratio of cell cycle.
【作者单位】：吉林医药学院生物化学教研室;吉林医药学院临床医学院;吉林医药学院药学院药物化学教研室;
【基金】：国家自然科学基金资助课题(21102055) 吉林省科技厅科技发展计划项目资助课题(20130101157JC) 吉林省教育厅“十二五”教育技术研究项目资助课题(2012-314,2014-371)
【分类号】：R737.33

【参考文献】