COX-2在VEGF-C介导的人宫颈癌细胞生物学行为中的作用研究

发布时间：2018-06-07 11:45

本文选题：血管内皮生长因子-C + 环氧合酶-　；参考：《华中科技大学学报(医学版)》2015年02期

【摘要】：目的检测血管内皮生长因子-C(VEGF-C)和环氧合酶-2(COX-2)在人宫颈癌细胞系中表达,探讨二者之间的关系及COX-2在VEGF-C介导的人宫颈癌细胞生物学行为中的作用。方法用Western blot方法检测VEGF-C和COX-2在HeLa、Siha、C33a和Caski等4株宫颈癌细胞中的表达;用特定浓度的COX-2特异性抑制剂NS398处理HeLa细胞,评估其对HeLa细胞的增殖、转移产生的影响。结果 VEGF-C和COX-2蛋白在4株宫颈癌细胞中均有表达,且在不同细胞株中二者表达强弱一致。用NS398处理HeLa细胞后,其VEGF-C蛋白表达明显降低,且细胞凋亡率增加。NS398可诱导HeLa细胞增殖活性降低(P0.05),且呈剂量依赖模式。Transwell实验结果显示NS398处理组细胞侵袭力为(25.45±3.67)%,较对照组(40.38±0.72)%明显减弱(P0.05)。结论 VEGF-C和COX-2均在人宫颈癌细胞中表达,且COX-2与VEGF-C表达之间有一定的相关性。COX-2可能是宫颈癌细胞中VEGF-C表达的一个调节者,其抑制剂NS398对于VEGF-C介导的肿瘤生长和转移有一定抑制作用。
[Abstract]:Objective to investigate the expression of vascular endothelial growth factor (VEGF-C) and cyclooxygenase-2 (COX-2) in human cervical cancer cell lines, and to explore the relationship between them and the role of COX-2 in the biological behavior of human cervical cancer cells mediated by VEGF-C. Methods Western blot method was used to detect the expression of VEGF-C and COX-2 in four cervical cancer cell lines, such as HeLa-Siha-C33a and Caski, and NS398, a specific inhibitor of COX-2, was used to treat HeLa cells to evaluate its effect on the proliferation and metastasis of HeLa cells. Results VEGF-C and COX-2 protein were expressed in all 4 cervical cancer cell lines, and the expression was consistent in different cell lines. After HeLa cells were treated with NS398, the expression of VEGF-C protein decreased significantly. The apoptosis rate increased. NS398 could induce the proliferation activity of HeLa cells to decrease P0.05G, and the results showed that the invasiveness of NS398 treated group was 25.45 卤3.67g, which was significantly lower than that of control group (40.38 卤0.72U%). Conclusion both VEGF-C and COX-2 are expressed in human cervical cancer cells, and there is some correlation between COX-2 and VEGF-C expression. COX-2 may be a regulator of VEGF-C expression in cervical cancer cells. Its inhibitor, NS398, has a certain inhibitory effect on VEGF-C mediated tumor growth and metastasis.
【作者单位】：武汉市中心医院中心实验室;武汉市中心医院临床检验科;
【基金】：湖北省自然科学基金资助项目(No.2013CFA060) 武汉市科技局资助项目(No.2014070504020245)
【分类号】：R737.33

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