多普勒彩超辐照对不同孕期胎鼠脑组织超微结构的影响

发布时间：2018-06-07 20:03

本文选题：胚胎发育 + 彩色多普勒超声检查　；参考：《第二军医大学学报》2015年09期

【摘要】：目的采用多普勒彩超辐照不同孕期大鼠,观察超声对胎鼠脑组织超微结构的影响。方法采用多普勒彩超照射不同孕期大鼠(孕12d、15d、18d)30min,仪器采用PREIRUS型彩色电脑声像仪,照射条件:EUP-LTM4探头,二维频率5.0 MHz,组织热指数(Tis)=0.4,机械指数(Micd)=1.2。照射后24h取胚胎脑组织标本,利用透射电镜(TEM)观察细胞器超微结构变化。结果经过多普勒彩超辐照30min后,孕期12d胎鼠神经元细胞器超微结构改变不明显,偶见线粒体嵴溶解,内质网未见明显扩张,细胞核形状较规则,胞质中有较小的空泡出现;孕期15d胎鼠神经元细胞器超微结构改变明显,细胞器损伤程度重于12d胎鼠;孕期18d胎鼠可见明显超微结构改变,内质网明显扩张,细胞核形态严重不规则,线粒体嵴溶解更明显,核周间隙扩张,胞质中出现较大空泡。结论多普勒彩超辐照对不同孕期胎鼠脑组织中细胞器超微结构的影响不同,胎鼠孕期越早,相同的辐照对其影响越小。
[Abstract]:Objective to observe the effect of Doppler ultrasound on the ultrastructure of fetal brain tissue. Methods Doppler ultrasound was used to irradiate rats of different gestation (12 days, 15 days, 18 days) for 30 minutes. The instrument was made with preirus color computer sonograph. The irradiation condition was as follows: 1. EUP-LTM4 probe, 2 D frequency 5.0 MHz, tissue heat index 0. 4, mechanical index 1. 2. The ultrastructural changes of organelle were observed by transmission electron microscope (TEM). Results after 30min was irradiated by Doppler ultrasound, the ultrastructure of neuronal organelle of fetal rat was not obvious at 12 days of gestation, the mitochondria crest was dissolved, the endoplasmic reticulum was not dilated, the shape of nucleus was regular, and there were small vacuoles in the cytoplasm. The ultrastructural changes of neuronal organelles were obvious in fetal mice at the 15th day of gestation, and the damage degree of organelle was more serious than that of the fetal mice at 12 days, and the ultrastructural changes, endoplasmic reticulum dilatation and serious irregular nuclear morphology were observed in the fetal mice on the 18th day of gestation. Mitochondrial cristolysis was more obvious, perinuclear space dilated, and large vacuoles appeared in the cytoplasm. Conclusion the effects of Doppler ultrasound irradiation on the ultrastructure of organelle in fetal brain tissue are different, and the earlier the fetal rat is, the less the same irradiation has on the ultrastructure of fetal brain tissue.
【作者单位】：山东医学高等专科学校附属医院超声科;第二军医大学基础部生物物理学教研室;
【分类号】：R714.5;R445.1

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