抗氧化剂对小鼠卵母细胞老化延缓作用的研究

发布时间：2018-06-08 04:54

本文选题：卵子衰老 + 氧化应激　；参考：《生殖与避孕》2015年05期

【摘要】：目的:探讨重金属耦合剂(乙二胺四乙酸和柠檬酸钠)与抗氧化剂(硫辛酸和乙酰肉碱)对小鼠卵子数目、线粒体和纺锤体老化的影响。方法:随机将C57BL/6雌性小鼠分为4组,重金属耦合剂组(A组)、抗氧化剂组(B组)、混合组(C组)和正常空白对照组(D组),每组48只。分别在饮用水中添加重金属耦合剂和/或抗氧化剂,并于饲养3个月、6个月、9个月和12个月后测定卵子数目、线粒体膜电位和纺锤体结构等指标。结果:小鼠卵子数和线粒体活性均随年龄增长而下降和减弱,纺锤体异常率随时间的延长而升高;在同一年龄阶段中,与A组和D组相比,B组和C组的卵子数目较多,长时间作用后,线粒体活性偏高,而纺锤体异常率偏低。结论:饮用水中添加抗氧化剂硫辛酸和乙酰肉碱可有延迟卵母细胞老化的作用。
[Abstract]:Aim: to investigate the effects of heavy metal coupling agents (ethylenediamine tetraacetic acid and sodium citrate) and antioxidants (lipoic acid and acetyl carnitine) on the aging of mouse eggs, mitochondria and spindles. Methods: C57BL / 6 female mice were randomly divided into 4 groups: heavy metal coupling group (group A), antioxidant group (group B), mixed group (group C) and normal control group (group D) with 48 rats in each group. Heavy metal coupling agents and / or antioxidants were added to drinking water, and egg numbers, mitochondrial membrane potential and spindle structure were measured after 3 months, 6 months, 9 months and 12 months of feeding. Results: the number of eggs and the activity of mitochondria decreased and decreased with age, and the abnormal rate of spindle increased with the increase of time, and the number of eggs in group B and group C was more than that in group A and group D in the same age period, the number of eggs in group B and group C was higher than that in group A and group D. The activity of mitochondria was higher and the abnormal rate of spindle was lower. Conclusion: the addition of antioxidant lipoic acid and acetyl carnitine in drinking water can delay oocyte aging.
【作者单位】：广州医科大学附属第三医院广东省普通高校生殖与遗传重点实验室;
【基金】：自然科学基金资助项目,项目号:81270745
【分类号】：R711.6

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