miR-21、miR-218在宫颈癌及癌前病变组织中的异常表达及与临床病理的关系

发布时间：2018-06-08 21:42

本文选题：miRNA-21 + miRNA-218　；参考：《广西医科大学》2014年硕士论文

【摘要】：目的：探讨miR-21、miR-218在宫颈癌及癌前组织中的表达及与高危型HPV感染和宫颈癌临床病理特征的关系。方法：（1）利用miRNA芯片技术筛查宫颈癌、宫颈上皮内瘤变中差异表达的miRNA，并用实时定量RT-PCR在108例宫颈组织标本中对miR-21、miR-218进行验证。（2）采用巢式多重PCR（NMPCR）技术对宫颈癌及癌前病变组织进行HPV分型检测。（3）利用统计学方法分析miR-21、miR-218在宫颈癌组织中的表达与高危型HPV感染及临床病理的关系。结果：（1）芯片结果显示宫颈癌及高级别鳞状上皮内病变（HSIL）组织中存在差异表达的miRNAs，，其中miR-218显示为最明显的下调，miR-21显示为最明显的上调，经实时定量RT-PCR验证结果一致。（2）宫颈癌及高级别鳞状上皮内病变中高危型HPV感染阳性率较高，均大于72%，以HPV-16感染为主，其次为HPV-18,HPV-45；高危型HPV感染与宫颈癌病理类型无明显相关性。（3）miR-21的表达与宫颈癌组织分化程度及高危型HPV感染有关，但与宫颈癌的FIGO分期、病理类型、淋巴结转移、脉管浸润等临床病理特征之间无明显相关性。（4）miR-218的表达与高危型HPV感染密切相关，但与宫颈癌的FIGO分期、病理类型、组织分化、淋巴结转移、脉管浸润等临床病理特征之间无明显相关性。结论：miR-21在宫颈癌组织中存在异常高表达，且与宫颈癌组织分化程度、高危型HPV感染相关；而miR-218在宫颈癌及癌前组织中则存在异常低表达，且与高危型HPV感染密切相关；miR-21、miR-218可能在宫颈癌及癌前病变中其起重要作用。
[Abstract]:Objective: to investigate the expression of miR-21 miR-218 in cervical carcinoma and precancerous tissues and its relationship with high-risk HPV infection and clinicopathological features of cervical cancer. Methods MiRNA microarray was used to screen cervical cancer. The differential expression of miRNAs in cervical intraepithelial neoplasia and the verification of miR-21 miR-218 in 108 cervical tissues by real-time quantitative RT-PCR were used to detect the HPV typing of cervical carcinoma and precancerous lesions by nested multiplex PCRNMPCRR). Methods the relationship between the expression of miR-21 miR-218 and high risk HPV infection and clinicopathology was analyzed. Results the microarray showed that there were differentially expressed miRNAss in cervical carcinoma and high grade squamous intraepithelial lesions (HSILs), in which miR-218 showed the most obvious down-regulation of miR-21 and the most obvious up-regulation of miR-21. The positive rate of high risk HPV infection in cervical carcinoma and high grade squamous intraepithelial lesions was higher than that in 722 cases. HPV-16 infection was the main infection. There was no significant correlation between high risk HPV infection and the pathological type of cervical cancer. The expression of miR-21 was related to the degree of cervical cancer differentiation and high risk HPV infection, but it was associated with Figo stage, pathological type and lymph node metastasis of cervical cancer. There was no significant correlation between vascular invasion and other clinicopathological features. The expression of miR-218 was closely related to high-risk HPV infection, but it was associated with Figo stage, pathological type, tissue differentiation, lymph node metastasis of cervical cancer. There was no significant correlation between the clinicopathological features such as vascular infiltration. Conclusion there is abnormal high expression of miR-21 in cervical carcinoma, which is related to the degree of differentiation and high risk HPV infection, but miR-218 is very low in cervical cancer and precancerous tissue. MiR-21 miR-218 may play an important role in cervical cancer and precancerous lesions.
【学位授予单位】：广西医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R737.33

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