miR-137过表达滋养细胞对血管内皮细胞生物学功能的调节作用

发布时间：2018-06-09 23:37

本文选题：miR- + 滋养细胞　；参考：《上海交通大学学报(医学版)》2017年10期

【摘要】：目的·探讨滋养细胞过表达miR-137后对血管内皮细胞生物学功能的影响。方法·构建Up-LV-miR-137和LV-miR-NC慢病毒载体,分别转染滋养细胞HTR-8/SVneo后通过PCR验证转染效率。成功转染后的滋养细胞与血管内皮细胞共培养,或以转染后滋养细胞培养基上清液培养血管内皮细胞,CCK8法、划痕试验和Transwell迁移试验分别检测内皮细胞的增殖活性、迁移能力和对单核细胞U937的招募能力。结果·成功获得miR-137过表达滋养细胞。CCK8细胞活性检测结果显示,与miR-137过表达滋养细胞共培养或以其培养基上清液培养,血管内皮细胞增殖活性显著降低,高糖环境下增殖活性进一步降低。划痕试验中,miR-137过表达细胞培养基使得内皮细胞划痕修复速度减慢。Transwell迁移试验结果发现,内皮细胞与miR-137过表达滋养细胞共培养后,该组单核细胞穿过小室的细胞数更多,趋化指数更高。结论·miR-137过表达滋养细胞可调节血管内皮细胞的生物学功能,降低增殖活性和迁移能力,增强其对单核细胞的招募能力。
[Abstract]:Objective to investigate the effect of overexpression of miR-137 in trophoblast on the biological function of vascular endothelial cells. Methods Up-LV-miR-137 and LV-miR-NC lentivirus vectors were constructed and transfected into trophoblast HTR-8 / SVneo, respectively. The transfection efficiency was verified by PCR. Successfully transfected trophoblast cells co-cultured with vascular endothelial cells, or cultured vascular endothelial cells by CCK8 medium supernatant of transfected trophoblastic medium, the proliferation activity of endothelial cells was detected by scratch test and Transwell migration test, respectively. Migration and recruitment of monocyte U937. Results the activity of miR-137 overexpression trophoblast. CCK8 cells were successfully obtained. The results showed that co-culture with miR-137 over-expressed trophoblastic cells or culture in supernatant of miR-137 culture medium significantly decreased the proliferation activity of vascular endothelial cells. The proliferative activity decreased further in high sugar environment. In scratch test, the culture medium of overexpression cells of miR-137 slowed down the rate of scar repair of endothelial cells. Transwell migration test showed that after co-culture of endothelial cells and miR-137 overexpression trophoblast cells, the number of monocytes passing through the chamber was more. The chemotaxis index is higher. Conclusion MiR-137 overexpression trophoblast can regulate the biological function of vascular endothelial cells, decrease proliferation activity and migration ability, and enhance its ability to recruit monocytes.
【作者单位】：上海交通大学附属第六人民医院妇产科;
【分类号】：R714.256

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