VEGF、MMP-2、MMP-9在子宫内膜息肉中的表达及其临床意义

发布时间：2018-06-12 13:30

本文选题：VEGF + MMP-2　；参考：《南华大学》2014年硕士论文

【摘要】：目的：检测血管内皮生长因子(vaseularendothelialgrowthfaetor,VEGF)、基质金属蛋白酶2(matrix metalloproteinase2, MMP-2)和基质金属蛋白酶9(matrixmetalloproteinase9, MMP-9)在子宫内膜息肉组织（复杂型增生及简单型增生）、息肉旁内膜组织及子宫正常内膜组织中的蛋白表达；探讨VEGF、MMP-2及MMP-9在子宫内膜息肉发病机制中的作用及其相关性。方法：标本收集：收集湘南学院附属医院2009年12月到2012年12月60例确诊为子宫内膜息肉组织样本为观察组，其中经确诊有30例为复杂性增生型子宫内膜息肉组织样本，30例为简单增生型子宫内膜息肉组织样本。然后再选择同期子宫内膜息肉旁的内膜组织样本30例为对照1组，选择正常子宫内膜组织样本30例为对照2组。免疫组化：取60例子宫内膜息肉、30例子宫内膜息肉旁的内膜组织及30例正常子宫内膜的组织石蜡标本，免疫组织化学染色，显微镜下观察VEGF、MMP-2及MMP-9蛋白表达。结果判断：根据染色细胞进行定性判断；采用阳性细胞百分率的半定量法来判定染色结果，在每例的染色结果中，阳性程度的判定采用积分方法：0~2分为阴性(-)；3~5分为弱阳性(+)；6~8分为阳性(++)；9~12分为强阳性(+++)。所有结果均由两位病理医师独立评定。统计分析：采用SPSS19.0统计软件包进行统计学分析。根据资料性质采用不同统计方法：两样本率的比较采用x2检验或fisher确切概率法；等级资料的比较采Mann-Whitney秩和检验。相关性分析采用spearman相关分析；计量资料采用t检验及方差分析。P0.05为统计学显著性意义标准。结果：（1）与对照1组和对照2组相比，子宫内膜息肉组包括复杂型增生息肉组和简单增生型息肉组VEGF蛋白表达增高，差异有统计学意义(P0.05)；在子宫内膜息肉组中，复杂型增生息肉组的VEGF阳性标本百分率高于简单增生型息肉组，差异具有统计学意义（x2=18.685，，P0.01），两组在染色分值上没有明显差异（x2=2.685，P0.05）。（2）MMP-2、MMP-9在复杂型增生息肉组和简单增生型息肉组的表达明显高于对照1组和对照2组，差异有统计学意义(P0.05)；MMP-9在对照1组和对照2组中的表达有明显差异(P0.05)，而复杂增生型子宫内膜息肉组的MMP-2、MMP-9阳性标本百分率为91.2%（27/30）、88.2%（26/30），明显高于简单增生型息肉组阳性标本百分率45.2%(14/30)、40.7%（12/30），而且其染色分值差异也具有统计学意义（x2=18.685，P0.01；x2=21.243，P0.05）。（3）在子宫内膜息肉组织中，VEGF、MMP-2、MMP-9三者表达呈正相关(F0.05)。结论：（1） VEGF、MMP-2、MMP-9三者在子宫内膜息肉组织高表达。（2）复杂增生型子宫内膜息肉VEGF、MMP-2、MMP-9蛋白表达高于简单增生型子宫内膜息肉。（3）VEGF、MMP-2、MMP-9三者在子宫内膜息肉蛋白表达呈正相关。
[Abstract]:Objective: to detect the expression of vascular endothelial growth factor (VEGF), matrix metalloproteinase (2(matrix) metalloproteinase 2 (MMP-2) and matrix metalloproteinase 9 (MMP-9) in endometrial polyps (complex hyperplasia and simple hyperplasia, peripolyp endometrial tissue and parenchyma). Protein expression in normal endometrial tissue; To investigate the role and correlation of VEGF MMP-2 and MMP-9 in the pathogenesis of endometrial polyps. Methods: specimens were collected from 60 cases of endometrial polyps from December 2009 to December 2012 in the affiliated Hospital of Xiangnan University. 30 cases of complex proliferative endometrial polyps were confirmed as simple proliferative endometrial polyps. Then 30 samples of endometrial tissue adjacent to endometrial polyps were selected as control group and 30 cases of normal endometrial tissue as control group. Immunohistochemical staining: the expressions of VEGF MMP-2 and MMP-9 were observed by immunohistochemical staining in 30 cases of endometrial tissues adjacent to endometrial polyps and 30 cases of normal endometrium. Results: qualitative judgment was made according to the staining cells, the semi-quantitative method of the percentage of positive cells was used to determine the staining results, and in each case, the positive degree was determined by integral method: 0 ~ 2 points were negative. In 3, 5 were weakly positive (P < 0. 05), 8 were positive (P < 0. 05), 12 were strongly positive (P < 0. 05). All results were assessed independently by two pathologists. Statistical analysis: SPSS 19.0 statistical software package was used for statistical analysis. According to the nature of the data, different statistical methods are used: the comparison of two sample rates is made by using x2 test or fisher exact probability method, and the comparison of grade data is based on Mann-Whitney rank sum test. Correlation analysis using spearman correlation analysis, measurement data using t test and variance analysis. P0.05 as the statistical significance standard. Results: compared with control group 1 and control group 2, the expression of VEGF protein in endometrial polyp group was significantly higher than that in control group (P 0.05), including complex hyperplasia polyp group and simple proliferative polyp group. The percentage of VEGF positive specimens in complex polyps was higher than that in simple proliferative polyps. There was no significant difference in the staining scores between the two groups. The expression of MMP-2MMP-9 in the complex hyperplasia polyp group and simple proliferative polyp group was significantly higher than that in the control group and the control group (P 0.05), and the difference was statistically significant (P 0.05). The expression of MMP-9 in control group 1 was significantly higher than that in control group 2 (P 0.05), while the positive rate of MMP-2 + MMP-9 in complex proliferative endometrial polyps was 91. 2%, 27 / 30 and 88. 2 / 30 respectively, which was significantly higher than that in simple proliferative polyp group (45.214 / 3040.7) and the positive rate of MMP-9 was 12 / 30%, and the positive rate of MMP-9 was significantly higher than that of simple proliferative polyp group (45.214 / 3040.7%), and the percentage of MMP-9 positive specimens was 91.2%. The difference of score was also statistically significant (P 0.01). The expression of VEGF, MMP-2, MMP-9 in endometrial polyps was positively correlated with that of F0. 05. Conclusion: (1) the expression of MMP-2 MMP-9 in endometrial polyps is higher than that in simple proliferative endometrial polyps, and the expression of MMP-9 is higher in endometrial polyps than that in simple proliferative endometrial polyps. The expression of MMP-9 and MMP-9 in endometrial polyps is higher than that in simple proliferative endometrial polyps, and the expression of MMP-9 in endometrial polyps is higher than that in simple proliferative endometrial polyps. The expression of endometrial polyp protein was positively correlated.
【学位授予单位】：南华大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R737.33

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